ArticleEukaryotic cell2004
Disordered cell integrity signaling caused by disruption of the kexB gene in Aspergillus oryzae.
Article in Eukaryotic cell, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 62 citations in OpenAlex.
- Engineering and application of a recombinant Aspergillus niger CECT 2907 tannase to enhance the antioxidant activity of black tea.Journal of the science of food and agriculture · 2026Article
- Amorphous-to-rodlet structural transition governs the interfacial functions ofFrontiers in microbiology · 2026Article
- FvKex2 is required for development, virulence, and mycotoxin production in Fusarium verticillioides.Applied microbiology and biotechnology · 2024Article
- Characterization of the Biosynthetic Gene Cluster for the Ribosomally Synthesized Cyclic Peptide Epichloëcyclins inJournal of agricultural and food chemistry · 2023Article
- Structural and Functional Analysis of Peptides Derived from KEX2-Processed Repeat Proteins in Agaricomycetes Using Reverse Genetics and Peptidomics.Microbiology spectrum · 2022Article
- Identification of Genes Involved in the Synthesis of the Fungal Cell Wall Component Nigeran and Regulation of Its Polymerization in AspergillusApplied and environmental microbiology · 2021Article
- Deletion of theMicroorganisms · 2020Article
- Dynamic Transcriptomic and Phosphoproteomic Analysis During Cell Wall Stress inMolecular & cellular proteomics : MCP · 2020Article
- Novel Inhibitory Function of theACS omega · 2019Article
- Heterologous Production of a Novel Cyclic Peptide Compound, KK-1, inFrontiers in microbiology · 2018Article
- Function and Biosynthesis of Cell Wall α-1,3-Glucan in Fungi.Journal of fungi (Basel, Switzerland) · 2017Review
- Characterization of Cell Wall α-1,3-Glucan-Deficient Mutants inJournal of applied glycoscience · 2017Article
- Genome sequence of Aspergillus luchuensis NBRC 4314.DNA research : an international journal for rapid publication of reports on genes and genomes · 2016Article
- Expression of ustR and the Golgi protease KexB are required for ustiloxin B biosynthesis in Aspergillus oryzae.AMB Express · 2016Article
- Kexin-like endoprotease KexB is required for N-glycan processing, morphogenesis and virulence in Aspergillus fumigatus.Fungal genetics and biology : FG & B · 2015Article
- Modified Cre-loxP recombination in Aspergillus oryzae by direct introduction of Cre recombinase for marker gene rescue.Applied and environmental microbiology · 2012Article
- Article
- Codon optimization increases steady-state mRNA levels in Aspergillus oryzae heterologous gene expression.Applied and environmental microbiology · 2008Article
- Processing of predicted substrates of fungal Kex2 proteinases from Candida albicans, C. glabrata, Saccharomyces cerevisiae and Pichia pastoris.BMC microbiology · 2008Article
- MpkA-Dependent and -independent cell wall integrity signaling in Aspergillus nidulans.Eukaryotic cell · 2007Article
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We isolated the kexB gene, which encodes a subtilisin-like processing enzyme, from a filamentous fungus, Aspergillus oryzae. To examine the physiological role of kexB in A. oryzae, we constructed a kexB disruptant (DeltakexB), which formed shrunken colonies with poor generation of conidia on Czapek-Dox (CD) agar plates and hyperbranched mycelia in CD liquid medium. The phenotypes of the DeltakexB strain were restored under high osmolarity in both solid and liquid culture conditions. We found that transcription of the mpkA gene, which encodes a putative mitogen-activated protein kinase involved in cell integrity signaling, was significantly higher in DeltakexB cells than in wild-type cells. The DeltakexB cells also contained higher levels of transcripts for cell wall-related genes encoding beta-1,3-glucanosyltransferase and chitin synthases, which is presumably attributable to cell integrity signaling through the increased gene expression of mpkA. As expected, constitutively increased levels of phosphorylated MpkA were observed in DeltakexB cells on the CD plate culture. High osmotic stress greatly downregulated the increased levels of both transcripts of mpkA and the phosphorylated form of MpkA in DeltakexB cells, concomitantly suppressing the morphological defects. These results suggest that the upregulation of transcription levels of mpkA and cell wall biogenesis genes in the DeltakexB strain is autoregulated by phosphorylated MpkA as the active form through cell integrity signaling. We think that KexB is required for precise proteolytic processing of sensor proteins in the cell integrity pathway or of cell wall-related enzymes under transcriptional control by the pathway and that the KexB defect thus induces disordered cell integrity signaling.
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