ArticleEukaryotic cell2004
Differential regulation of ceramide synthase components LAC1 and LAG1 in Saccharomyces cerevisiae.
Article in Eukaryotic cell, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 63 citations in OpenAlex.
- Functional analysis of Candida albicans Cdr1 through homologous and heterologous expression studies.FEMS yeast research · 2025Review
- Phenotype to genotype inCurrent research in microbial sciences · 2022Article
- The Ceramide Synthase Subunit Lac1 Regulates Cell Growth and Size in Fission Yeast.International journal of molecular sciences · 2021Article
- Article
- Cytotoxicity of 1-deoxysphingolipid unraveled by genome-wide genetic screens and lipidomics inMolecular biology of the cell · 2019Article
- Centromeric non-coding RNA as a hidden epigenetic factor of the point centromere.Current genetics · 2019Review
- Unveiling the transcriptional control of pleiotropic drug resistance in Saccharomyces cerevisiae: Contributions of André Goffeau and his group.Yeast (Chichester, England) · 2019Review
- Point centromere activity requires an optimal level of centromeric noncoding RNA.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- The Regulation of Cbf1 by PAS Kinase Is a Pivotal Control Point for LipogenesisG3 (Bethesda, Md.) · 2019Article
- Candida albicans gains azole resistance by altering sphingolipid composition.Nature communications · 2018Article
- Distinct roles of the 7-transmembrane receptor protein Rta3 in regulating the asymmetric distribution of phosphatidylcholine across the plasma membrane and biofilm formation in Candida albicans.Cellular microbiology · 2017Article
- PP2A Controls Genome Integrity by Integrating Nutrient-Sensing and Metabolic Pathways with the DNA Damage Response.Molecular cell · 2017Article
- Mitochondria to nucleus signaling and the role of ceramide in its integration into the suite of cell quality control processes during aging.Ageing research reviews · 2015Review
- Control of Plasma Membrane Permeability by ABC Transporters.Eukaryotic cell · 2015Article
- Sphingolipids and mitochondrial function in budding yeast.Biochimica et biophysica acta · 2014Article
- A comprehensive protein-protein interactome for yeast PAS kinase 1 reveals direct inhibition of respiration through the phosphorylation of Cbf1.Molecular biology of the cell · 2014Article
- Activation of the pleiotropic drug resistance pathway can promote mitochondrial DNA retention by fusion-defective mitochondria in Saccharomyces cerevisiae.G3 (Bethesda, Md.) · 2014Article
- The retrograde response: a conserved compensatory reaction to damage from within and from without.Progress in molecular biology and translational science · 2014Review
- Multidrug resistance in fungi: regulation of transporter-encoding gene expression.Frontiers in physiology · 2014Review
- TEAK: topology enrichment analysis framework for detecting activated biological subpathways.Nucleic acids research · 2013Article
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Authors and funding
5 authors at 4 institutions in 3 countries.
Funding
Abstract
In Saccharomyces cerevisiae, the essential ceramide synthase reaction requires the presence of one of a homologous pair of genes, LAG1 and LAC1. Mutants that lack both of these genes cannot produce ceramide and exhibit a striking synthetic growth defect. While the regulation of ceramide production is critical for the control of proliferation and for stress tolerance, little is known of the mechanisms that ensure proper control of this process. The data presented here demonstrate that the pleiotropic drug resistance (Pdr) regulatory pathway regulates the transcription of multiple genes encoding steps in sphingolipid biosynthesis, including LAC1. The zinc cluster transcriptional activators Pdr1p and Pdr3p bind to Pdr1p/Pdr3p-responsive elements (PDREs) in the promoters of Pdr pathway target genes. LAC1 contains a single PDRE in its promoter, but notably, LAG1 does not. Reporter gene, Northern blot, and Western blot assays indicated that the expression level of Lac1p is approximately three times that of Lag1p. Detailed analyses of the LAC1 promoter demonstrated that transcription of this gene is inhibited by the presence of the transcription factor Cbf1p and the anaerobic repressor Rox1p. LAG1 transcription was also elevated in cbf1Delta cells, indicating at least one common regulatory input. Although a hyperactive Pdr pathway altered the profile of sphingolipids produced, the loss of either LAC1 or LAG1 alone failed to produce further changes. Two other genes involved in sphingolipid biosynthesis (LCB2 and SUR2) were found to contain PDREs in their promoters and to be induced by the Pdr pathway. These data demonstrate extensive coordinate control of sphingolipid biosynthesis and multidrug resistance in yeast.
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