ArticleNature biotechnology2004
5'-end SAGE for the analysis of transcriptional start sites.
Article in Nature biotechnology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
46 citing papers in PubMed.
- Morphogenesis, starvation, and light responses in a mushroom-forming fungus revealed by long-read sequencing and extensive expression profiling.Cell genomics · 2025Article
- Quantitative analysis of transcription start site selection reveals control by DNA sequence, RNA polymerase II activity and NTP levels.Nature structural & molecular biology · 2024Article
- Global approaches for profiling transcription initiation.Cell reports methods · 2021Review
- Computational annotation of miRNA transcription start sites.Briefings in bioinformatics · 2021Review
- Alternative transcription start sites of the enolase-encoding gene enoA are stringently used in glycolytic/gluconeogenic conditions in Aspergillus oryzae.Current genetics · 2020Article
- Genome-wide DNA methylation analysis of senescence in repetitively infected memory cytotoxic T lymphocytes.Immunology · 2018Article
- Maser: one-stop platform for NGS big data from analysis to visualization.Database : the journal of biological databases and curation · 2018Article
- Identifying transcription start sites and active enhancer elements using BruUV-seq.Scientific reports · 2015Article
- EXPRSS: an Illumina based high-throughput expression-profiling method to reveal transcriptional dynamics.BMC genomics · 2014Article
- Transcriptome analysis of the filamentous fungus Aspergillus nidulans directed to the global identification of promoters.BMC genomics · 2013Article
- 5'-Serial Analysis of Gene Expression studies reveal a transcriptomic switch during fruiting body development in Coprinopsis cinerea.BMC genomics · 2013Article
- Metazoan promoters: emerging characteristics and insights into transcriptional regulation.Nature reviews. Genetics · 2012Review
- Recognition and suppression of transfected plasmids by protein ZNF511-PRAP1, a potential molecular barrier to transgene expression.Molecular therapy : the journal of the American Society of Gene Therapy · 2011Article
- Predicting promoter activities of primary human DNA sequences.Nucleic acids research · 2011Article
- Genome-wide characterization of transcriptional start sites in humans by integrative transcriptome analysis.Genome research · 2011Article
- Regulated post-transcriptional RNA cleavage diversifies the eukaryotic transcriptome.Genome research · 2010Article
- Article
- Characterization of transcription start sites of putative non-coding RNAs by multifaceted use of massively paralleled sequencer.DNA research : an international journal for rapid publication of reports on genes and genomes · 2010Article
- Genomics and bioinformatics resources for crop improvement.Plant & cell physiology · 2010Review
- Quantification of the yeast transcriptome by single-molecule sequencing.Nature biotechnology · 2009Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Identification of the mRNA start site is essential in establishing the full-length cDNA sequence of a gene and analyzing its promoter region, which regulates gene expression. Here we describe the development of a 5'-end serial analysis of gene expression (5' SAGE) that can be used to globally identify transcriptional start sites and the frequency of individual mRNAs. Of the 25,684 5' SAGE tags in the HEK293 human cell library, 19,893 matched to the human genome. Among 15,448 tags in one locus of the genome, 85.8%-96.1% of the 5' SAGE tags were assigned within -500 to +200 nt of mRNA start sites using the RefSeq, UniGene and DBTSS databases. This technique should facilitate 5'-end transcriptome analysis in a variety of cells and tissues.
Indexed as
Identifiers
15300261What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.