ArticleNature biotechnology2004
Post-integration stabilization of a transposon vector by terminal sequence deletion in Drosophila melanogaster.
Article in Nature biotechnology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed.
- Transposon-based genome editing of industrial microorganisms: advances, challenges, and prospects.Synthetic and systems biotechnology · 2026Review
- Unpredictable recombination of PB transposon in Silkworm: a potential risk.Molecular genetics and genomics : MGG · 2021Article
- Improvement on the genetic engineering of an invasive agricultural pest insect, the cherry vinegar fly, Drosophila suzukii.BMC genetics · 2020Article
- AnG3 (Bethesda, Md.) · 2018Article
- Remobilizing deleted piggyBac vector post-integration for transgene stability in silkworm.Molecular genetics and genomics : MGG · 2015Article
- An efficient strategy for producing a stable, replaceable, highly efficient transgene expression system in silkworm, Bombyx mori.Scientific reports · 2015Article
- Site-specific cassette exchange systems in the Aedes aegypti mosquito and the Plutella xylostella moth.PloS one · 2015Article
- Not all GMOs are crop plants: non-plant GMO applications in agriculture.Transgenic research · 2014Review
- Site-specific, TALENs-mediated transformation of Bombyx mori.Insect biochemistry and molecular biology · 2014Article
- Establishment of transgenic lines for jumpstarter method using a composite transposon vector in the ladybird beetle, Harmonia axyridis.PloS one · 2014Article
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- A Functional Comparison of the 3xP3 Promoter by Recombinase-Mediated Cassette Exchange in Drosophila and a Tephritid Fly, Anastrepha suspensa.G3 (Bethesda, Md.) · 2013Article
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- Strategy for enhanced transgenic strain development for embryonic conditional lethality in Anastrepha suspensa.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
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- A new powerful method for site-specific transgene stabilization based on chromosomal double-strand break repair.PloS one · 2011Article
- Development of transgenic strains for the biological control of the Mexican fruit fly, Anastrepha ludens.Genetica · 2011Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Germline transformation systems for nearly 20 insect species have been derived from transposable elements, allowing the development of transgenic insects for basic and applied studies. These systems use a defective nonautonomous vector that results in stable vector integrations after the disappearance of transiently provided transposase helper plasmid, which is essential to maintain true breeding lines and consistent transgene expression that would otherwise be lost after vector remobilization. The risk of remobilization by an unintended transposase source has so far not been a concern for laboratory studies, but the prospective use of millions of transgenic insects in biocontrol programs will likely increase the risk, therefore making this a critical issue for the ecological safety of field release programs. Here we describe an efficient method that deletes a terminal repeat sequence of a transposon vector after genomic integration. This procedure prevents transposase-mediated remobilization of the other terminal sequence and associated genes, ensuring their genomic stability.
Indexed as
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.