ArticlePlant physiology2004
Proteome reference maps of vegetative tissues in pea. An investigation of nitrogen mobilization from leaves during seed filling.
Article in Plant physiology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 139 citations in OpenAlex.
- In-depth assembly of organ and development dissected Picrorhiza kurroa proteome map using mass spectrometry.BMC plant biology · 2021Article
- Plant science's next top models.Annals of botany · 2020Review
- Proteomic Analysis of Tung Tree (Vernicia fordii) Oilseeds during the Developmental Stages.Molecules (Basel, Switzerland) · 2016Article
- Extracellular Matrix Proteome and Phosphoproteome of Potato Reveals Functionally Distinct and Diverse Canonical and Non-Canonical Proteoforms.Proteomes · 2016Article
- Integrating transcriptomic and metabolomic analysis to understand natural leaf senescence in sunflower.Plant biotechnology journal · 2016Article
- Emerging Genomic Tools for Legume Breeding: Current Status and Future Prospects.Frontiers in plant science · 2016Review
- Proteomic Analysis Reveals Different Involvement of Embryo and Endosperm Proteins during Aging of Yliangyou 2 Hybrid Rice Seeds.Frontiers in plant science · 2016Article
- Secretome analysis of chickpea reveals dynamic extracellular remodeling and identifies a Bet v1-like protein, CaRRP1 that participates in stress response.Scientific reports · 2015Article
- The QQS orphan gene of Arabidopsis modulates carbon and nitrogen allocation in soybean.Plant biotechnology journal · 2015Article
- Proteomics and Metabolomics: Two Emerging Areas for Legume Improvement.Frontiers in plant science · 2015Review
- Pre-fractionation strategies to resolve pea (Pisum sativum) sub-proteomes.Frontiers in plant science · 2015Review
- Proteome Analysis of Poplar Seed Vigor.PloS one · 2015Article
- A systems biology approach toward understanding seed composition in soybean.BMC genomics · 2015Article
- Towards systems biological understanding of leaf senescence.Plant molecular biology · 2013Review
- Proteome Analysis of Rice (Oryza sativa L.) Mutants Reveals Differentially Induced Proteins during Brown Planthopper (Nilaparvata lugens) Infestation.International journal of molecular sciences · 2013Article
- A systemic proteomic analysis of Populus chloroplast by using shotgun method.Molecular biology reports · 2011Article
- Article
- Metabolic adaptation in transplastomic plants massively accumulating recombinant proteins.PloS one · 2011Article
- Proteome analysis of embryogenic cell suspensions of cowpea (Vigna unguiculata).Plant cell reports · 2007Article
- Developmental genes have pleiotropic effects on plant morphology and source capacity, eventually impacting on seed protein content and productivity in pea.Plant physiology · 2007Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A proteomic approach was used to analyze protein changes during nitrogen mobilization (N mobilization) from leaves to filling seeds in pea (Pisum sativum). First, proteome reference maps were established for mature leaves and stems. They displayed around 190 Coomassie Blue-stained spots with pIs from 4 to 7. A total of 130 spots were identified by mass spectrometry as corresponding to 80 different proteins implicated in a variety of cellular functions. Although the leaf proteome map contained more abundant spots, corresponding to proteins involved in energy/carbon metabolism, than the stem map, their comparison revealed a highly similar protein profile. Second, the leaf proteome map was used to analyze quantitative variations in leaf proteins during N mobilization. Forty percent of the spots showed significant changes in their relative abundance in the total protein extract. The results confirmed the importance of Rubisco as a source of mobilizable nitrogen, and suggested that in pea leaves the rate of degradation of Rubisco may vary throughout N mobilization. Correlated with the loss of Rubisco was an increase in relative abundance of chloroplastic protease regulatory subunits. Concomitantly, the relative abundance of some proteins related to the photosynthetic apparatus (Rubisco activase, Rubisco-binding proteins) and of several chaperones increased. A role for these proteins in the maintenance of a Rubisco activation state and in the PSII repair during the intense proteolytic activity within the chloroplasts was proposed. Finally, two 14-3-3-like proteins, with a potential regulatory role, displayed differential expression patterns during the massive remobilization of nitrogen.
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