ArticlePlant physiology2004
AtPng1p. The first plant transglutaminase.
Article in Plant physiology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 82 citations in OpenAlex.
- At the core of global bioenergy: polyamines and transglutaminases in chloroplasts.Amino acids · 2026Review
- Fungal transglutaminase domain-containing proteins are involved in hyphal protection at the septal pore against wounding.Molecular biology of the cell · 2023Article
- Review
- Comprehensive Analysis of the Structure and Function of Peptide:N-Glycanase 1 and Relationship with Congenital Disorder of Deglycosylation.Nutrients · 2022Review
- Overexpression of Transglutaminase from Cucumber in Tobacco Increases Salt Tolerance through Regulation of Photosynthesis.International journal of molecular sciences · 2019Article
- TGase positively regulates photosynthesis via activation of Calvin cycle enzymes in tomato.Horticulture research · 2019Article
- Optimization of recombinantFood science and biotechnology · 2017Article
- Polyamine catabolism adds fuel to leaf senescence.Amino acids · 2017Review
- Hydrogen Peroxide and Polyamines Act as Double Edged Swords in Plant Abiotic Stress Responses.Frontiers in plant science · 2016Review
- Dark-induced senescence of barley leaves involves activation of plastid transglutaminases.Amino acids · 2015Article
- Senescence and programmed cell death in plants: polyamine action mediated by transglutaminase.Frontiers in plant science · 2014Review
- Expression of soluble recombinant transglutaminase from Zea mays in Pichia pastoris.World journal of microbiology & biotechnology · 2013Article
- Distribution of transglutaminase in pear pollen tubes in relation to cytoskeleton and membrane dynamics.Plant physiology · 2013Article
- Regulation of Pollen Tube Growth by Transglutaminase.Plants (Basel, Switzerland) · 2013Review
- Identification and characterization of peptide: N-glycanase from Dictyostelium discoideum.BMC biochemistry · 2012Article
- Evidence for an essential deglycosylation-independent activity of PNGase in Drosophila melanogaster.PloS one · 2010Article
- Transglutaminases and their substrates in kinetin-stimulated etioplast-to-chloroplast transformation in cucumber cotyledons.Protoplasma · 2008Article
- Review
- Transglutaminases: widespread cross-linking enzymes in plants.Annals of botany · 2008Article
- The acropetal wave of developmental cell death of tobacco corolla is preceded by activation of transglutaminase in different cell compartments.Plant physiology · 2007Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Studies have revealed in plant chloroplasts, mitochondria, cell walls, and cytoplasm the existence of transglutaminase (TGase) activities, similar to those known in animals and prokaryotes having mainly structural roles, but no protein has been associated to this type of activity in plants. A recent computational analysis has shown in Arabidopsis the presence of a gene, AtPng1p, which encodes a putative N-glycanase. AtPng1p contains the Cys-His-Asp triad present in the TGase catalytic domain. AtPng1p is a single gene expressed ubiquitously in the plant but at low levels in all light-assayed conditions. The recombinant AtPng1p protein could be immuno-detected using animal TGase antibodies. Furthermore, western-blot analysis using antibodies raised against the recombinant AtPng1p protein have lead to its detection in microsomal fraction. The purified protein links polyamines-spermine (Spm) > spermidine (Spd) > putrescine (Put)-and biotin-cadaverine to dimethylcasein in a calcium-dependent manner. Analyses of the gamma-glutamyl-derivatives revealed that the formation of covalent linkages between proteins and polyamines occurs via the transamidation of gamma-glutamyl residues of the substrate, confirming that the AtPng1p gene product acts as a TGase. The Ca(2+)- and GTP-dependent cross-linking activity of the AtPng1p protein can be visualized by the polymerization of bovine serum albumine, obtained, like the commercial TGase, at basic pH and in the presence of dithiotreitol. To our knowledge, this is the first reported plant protein, characterized at molecular level, showing TGase activity, as all its parameters analyzed so far agree with those typically exhibited by the animal TGases.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.