ArticlePlant physiology2004
The lys5 mutations of barley reveal the nature and importance of plastidial ADP-Glc transporters for starch synthesis in cereal endosperm.
Article in Plant physiology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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30 citing papers in PubMed, 124 citations in OpenAlex.
- HvPHO1 Controls a Glucose 1-Phosphate-SnRK1 Signalling Module That Coordinates Starch Accumulation in Barley Endosperm.Plant biotechnology journal · 2026Article
- Breeding of Fukumi Fiber, a new six-rowed waxy hull-less barley cultivar containing high levels of β-glucan with a proanthocyanidin-free gene.Breeding science · 2025Article
- Development of a KASP marker set for high-throughput genotyping in Japanese barley breeding programs with various end-use purposes.Breeding science · 2025Article
- Structure, function, and evolution of plant ADP-glucose pyrophosphorylase.Plant molecular biology · 2022Review
- Morphological and Molecular Characterization of Some Egyptian Six-Rowed Barley (Plants (Basel, Switzerland) · 2021Article
- A novel mutant with triple the dietary fiber content in white rice.Breeding science · 2021Article
- Proteome and Nutritional Shifts Observed in Hordein Double-Mutant Barley Lines.Frontiers in plant science · 2021Article
- A review of starch biosynthesis in cereal crops and its potential breeding applications in rice (PeerJ · 2021Article
- Identification and characterization of mRNAs and lncRNAs of a barley shrunken endosperm mutant using RNA-seq.Genetica · 2020Article
- Overexpression of HvCslF6 in barley grain alters carbohydrate partitioning plus transfer tissue and endosperm development.Journal of experimental botany · 2020Article
- Characterisation of Cellulose Synthase Like F6 (Frontiers in plant science · 2020Article
- Insight Into the Prospects for the Improvement of Seed Starch in Legume-A Review.Frontiers in plant science · 2019Review
- Carbohydrate reserves and seed development: an overview.Plant reproduction · 2018Review
- From metabolome to phenotype: GC-MS metabolomics of developing mutant barley seeds reveals effects of growth, temperature and genotype.Scientific reports · 2017Article
- OsBT1 encodes an ADP-glucose transporter involved in starch synthesis and compound granule formation in rice endosperm.Scientific reports · 2017Article
- Analysis of the Rice ADP-Glucose Transporter (OsBT1) Indicates the Presence of Regulatory Processes in the Amyloplast Stroma That Control ADP-Glucose Flux into Starch.Plant physiology · 2016Article
- Article
- Genetic Diversity and Genome Wide Association Study of β-Glucan Content in Tetraploid Wheat Grains.PloS one · 2016Article
- Laser microdissection-based gene expression analysis in the aleurone layer and starchy endosperm of developing rice caryopses in the early storage phase.Rice (New York, N.Y.) · 2015Article
- AGPase: its role in crop productivity with emphasis on heat tolerance in cereals.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2015Review
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6 authors at 3 institutions in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Much of the ADP-Glc required for starch synthesis in the plastids of cereal endosperm is synthesized in the cytosol and transported across the plastid envelope. To provide information on the nature and role of the plastidial ADP-Glc transporter in barley (Hordeum vulgare), we screened a collection of low-starch mutants for lines with abnormally high levels of ADP-Glc in the developing endosperm. Three independent mutants were discovered, all of which carried mutations at the lys5 locus. Plastids isolated from the lys5 mutants were able to synthesize starch at normal rates from Glc-1-P but not from ADP-Glc, suggesting a specific lesion in the transport of ADP-Glc across the plastid envelope. The major plastidial envelope protein was purified, and its sequence showed it to be homologous to the maize (Zea mays) ADP-Glc transporter BRITTLE1. The gene encoding this protein in barley, Hv.Nst1, was cloned, sequenced, and mapped. Like lys5, Hv.Nst1 lies on chromosome 6(6H), and all three of the lys5 alleles that were examined were shown to carry lesions in Hv.Nst1. Two of the identified mutations in Hv.Nst1 lead to amino acid substitutions in a domain that is conserved in all members of the family of carrier proteins to which Hv.NST1 belongs. This strongly suggests that Hv.Nst1 lies at the Lys5 locus and encodes a plastidial ADP-Glc transporter. The low-starch phenotype of the lys5 mutants shows that the ADP-Glc transporter is required for normal rates of starch synthesis. This work on Hv.NST1, together with the earlier work on BRITTLE1, suggests that homologous transporters are probably present in the endosperm of all cereals.
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