ArticleBiophysical journal2004
The elasticity of single titin molecules using a two-bead optical tweezers assay.
Article in Biophysical journal, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed, 91 citations in OpenAlex.
- Correlating fluorescence microscopy, optical and magnetic tweezers to study single chiral biopolymers such as DNA.Nature communications · 2024Article
- Single-molecule and super-resolved imaging deciphers membrane behavior of onco-immunogenic CCR5.iScience · 2022Article
- RecA and RecB: probing complexes of DNA repair proteins with mitomycin C in liveJournal of the Royal Society, Interface · 2022Article
- Critical roles for EGFR and EGFR-HER2 clusters in EGF binding of SW620 human carcinoma cells.Journal of the Royal Society, Interface · 2022Article
- A nanoscale DNA force spectrometer capable of applying tension and compression on biomolecules.Nucleic acids research · 2021Article
- Measurement of hindered diffusion in complex geometries for high-speed studies of single-molecule forces.Scientific reports · 2021Article
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- Stator Dynamics Depending on Sodium Concentration in Sodium-Driven Bacterial Flagellar Motors.Frontiers in microbiology · 2021Article
- Single-molecule optical microscopy of protein dynamics and computational analysis of images to determine cell structure development in differentiatingComputational and structural biotechnology journal · 2020Article
- Single-molecule live cell imaging of Rep reveals the dynamic interplay between an accessory replicative helicase and the replisome.Nucleic acids research · 2019Article
- Single-Organelle Quantification Reveals Stoichiometric and Structural Variability of Carboxysomes Dependent on the Environment.The Plant cell · 2019Article
- Elasticity of individual protocadherin 15 molecules implicates tip links as the gating springs for hearing.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Article
- Automated Dielectrophoretic Tweezers-Based Force Spectroscopy System in a Microfluidic Device.Sensors (Basel, Switzerland) · 2017Article
- Single-molecule fluorescence microscopy review: shedding new light on old problems.Bioscience reports · 2017Review
- Article
- Computing Average Passive Forces in Sarcomeres in Length-Ramp Simulations.PLoS computational biology · 2016Article
- A novel three-filament model of force generation in eccentric contraction of skeletal muscles.PloS one · 2015Article
- Molecular basis of the mechanical hierarchy in myomesin dimers for sarcomere integrity.Biophysical journal · 2014Article
- Transcriptional regulation and alternative splicing cooperate in muscle fiber-type specification in flies and mammals.Experimental cell research · 2014Review
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Titin is responsible for the passive elasticity of the muscle sarcomere. The mechanical properties of skeletal and cardiac muscle titin were characterized in single molecules using a novel dual optical tweezers assay. Antibody pairs were attached to beads and used to select the whole molecule, I-band, A-band, a tandem-immunoglobulin (Ig) segment, and the PEVK region. A construct from the PEVK region expressing >25% of the full-length skeletal muscle isoform was chemically conjugated to beads and similarly characterized. By elucidating the elasticity of the different regions, we showed directly for the first time, to our knowledge, that two entropic components act in series in the skeletal muscle titin I-band (confirming previous speculations), one associated with tandem-immunoglobulin domains and the other with the PEVK region, with persistence lengths of 2.9 nm and 0.76 nm, respectively (150 mM ionic strength, 22 degrees C). Novel findings were: the persistence length of the PEVK component rose (0.4-2.7 nm) with an increase in ionic strength (15-300 mM) and fell (3.0-0.3 nm) with a temperature increase (10-60 degrees C); stress-relaxation in 10-12-nm steps was observed in the PEVK construct and hysteresis in the native PEVK region. The region may not be a pure random coil, as previously thought, but contains structured elements, possibly with hydrophobic interactions.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.