ArticleBiophysical journal2004
Effects of Kv1.2 intracellular regions on activation of Kv2.1 channels.
Article in Biophysical journal, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Kv2.1 channels play opposing roles in regulating membrane potential, CaProceedings of the National Academy of Sciences of the United States of America · 2020Article
- The Inhibitory Effects of Ca2+ Channel Blocker Nifedipine on Rat Kv2.1 Potassium Channels.PloS one · 2015Article
- Cytoplasmic domains and voltage-dependent potassium channel gating.Frontiers in pharmacology · 2012Article
- Role of the S4-S5 linker in CNG channel activation.Biophysical journal · 2010Article
- Rearrangements in the relative orientation of cytoplasmic domains induced by a membrane-anchored protein mediate modulations in Kv channel gating.The Journal of biological chemistry · 2009Article
- Selective interaction of syntaxin 1A with KCNQ2: possible implications for specific modulation of presynaptic activity.PloS one · 2009Article
- Roles of surface residues of intracellular domains of heag potassium channels.European biophysics journal : EBJ · 2009Article
- Intracellular regions of potassium channels: Kv2.1 and heag.European biophysics journal : EBJ · 2009Review
- Dynamic coupling of voltage sensor and gate involved in closed-state inactivation of kv4.2 channels.The Journal of general physiology · 2009Article
- Kv channel gating requires a compatible S4-S5 linker and bottom part of S6, constrained by non-interacting residues.The Journal of general physiology · 2008Article
- Interdomain cytoplasmic interactions govern the intracellular trafficking, gating, and modulation of the Kv2.1 channel.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2008Article
- Single particle image reconstruction of the human recombinant Kv2.1 channel.Biophysical journal · 2008Article
- The axon-dendrite targeting of Kv3 (Shaw) channels is determined by a targeting motif that associates with the T1 domain and ankyrin G.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2007Article
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Authors and funding
6 authors.
Funding
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Abstract
Depolarizing voltage steps activate voltage-dependent K(+) (Kv) channels by moving the voltage sensor, which triggers a coupling reaction leading to the opening of the pore. We constructed chimeric channels in which intracellular regions of slowly activating Kv2.1 channels were replaced by respective regions of rapidly activating Kv1.2 channels. Substitution of either the N-terminus, S4-S5 linker, or C-terminus generated chimeric Kv2.1/1.2 channels with a paradoxically slow and approximately exponential activation time course consisting of a fast and a slow component. Using combined chimeras, each of these Kv1.2 regions further slowed activation at the voltage of 0 mV, irrespective of the nature of the other two regions, whereas at the voltage of 40 mV both slowing and accelerating effects were observed. These results suggest voltage-dependent interactions of the three intracellular regions. This observation was quantified by double-mutant cycle analysis. It is concluded that interactions between N-terminus, S4-S5 linker, and/or C-terminus modulate the activation time course of Kv2.1 channels and that part of these interactions is voltage dependent.
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