ArticleBiophysical journal2004
Voltage-gated rearrangements associated with differential beta-subunit modulation of the L-type Ca(2+) channel inactivation.
Article in Biophysical journal, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 31 citations in OpenAlex.
- How CaThe Journal of general physiology · 2019Article
- Site-Directed Fluorescence Approaches for Dynamic Structural Biology of Membrane Peptides and Proteins.Frontiers in molecular biosciences · 2019Review
- Lead poisoning: acute exposure of the heart to lead ions promotes changes in cardiac function and Cav1.2 ion channels.Biophysical reviews · 2017Review
- Rational strategy to stop arrhythmias: Early afterdepolarizations and L-type Ca2+ current.The Journal of general physiology · 2015Article
- Application of fluorescence resonance energy transfer in protein studies.Journal of molecular structure · 2014Article
- Patch-clamp fluorometry: electrophysiology meets fluorescence.Biophysical journal · 2014Review
- Three-dimensional localization of the α and β subunits and of the II-III loop in the skeletal muscle L-type Ca2+ channel.The Journal of biological chemistry · 2012Article
- Molecular Determinants of Cav1.2 Calcium Channel Inactivation.ISRN molecular biology · 2012Review
- Truncation of murine CaV1.2 at Asp-1904 results in heart failure after birth.The Journal of biological chemistry · 2011Article
- Fluorescence anisotropy and resonance energy transfer: powerful tools for measuring real time protein dynamics in a physiological environment.Current opinion in pharmacology · 2010Review
- Inactivation of L-type calcium channels is determined by the length of the N terminus of mutant beta(1) subunits.Pflugers Archiv : European journal of physiology · 2010Article
- Effect of Ca(v)beta subunits on structural organization of Ca(v)1.2 calcium channels.PloS one · 2009Article
- Accelerated inactivation of the L-type calcium current due to a mutation in CACNB2b underlies Brugada syndrome.Journal of molecular and cellular cardiology · 2009Article
- Arachidonic acid inhibition of L-type calcium (CaV1.3b) channels varies with accessory CaVbeta subunits.The Journal of general physiology · 2009Article
- Calmodulin-dependent gating of Ca(v)1.2 calcium channels in the absence of Ca(v)beta subunits.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- New Determinant for the CaVbeta2 subunit modulation of the CaV1.2 calcium channel.The Journal of biological chemistry · 2008Article
- Regulation of maximal open probability is a separable function of Ca(v)beta subunit in L-type Ca2+ channel, dependent on NH2 terminus of alpha1C (Ca(v)1.2alpha).The Journal of general physiology · 2006Article
- Vascular calcium channels and high blood pressure: pathophysiology and therapeutic implications.Vascular pharmacology · 2006Review
- Identification of plasma membrane macro- and microdomains from wavelet analysis of FRET microscopy.Biophysical journal · 2005Article
- Selective fluorophore-assisted light inactivation of voltage-gated calcium channels.Channels (Austin, Tex.)Article
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Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
Abstract
Auxiliary beta-subunits bound to the cytoplasmic alpha(1)-interaction domain of the pore-forming alpha(1C)-subunit are important modulators of voltage-gated Ca(2+) channels. The underlying mechanisms are not yet well understood. We investigated correlations between differential modulation of inactivation by beta(1a)- and beta(2)- subunits and structural responses of the channel to transition into distinct functional states. The NH(2)-termini of the alpha(1C)- and beta-subunits were fused with cyan or yellow fluorescent proteins, and functionally coexpressed in COS1 cells. Fluorescence resonance energy transfer (FRET) between them or with membrane-trapped probes was measured in live cells under voltage clamp. It was found that in the resting state, the tagged NH(2)-termini of the alpha(1C)- and beta-subunit fluorophores are separated. Voltage-dependent inactivation generates strong FRET between alpha(1C) and beta(1a) suggesting mutual reorientation of the NH(2)-termini, but their distance vis-à-vis the plasma membrane is not appreciably changed. These voltage-gated rearrangements were substantially reduced when the beta(1a)-subunit was replaced by beta(2). Differential beta-subunit modulation of inactivation and of FRET between alpha(1C) and beta were eliminated by inhibition of the slow inactivation. Thus, differential beta-subunit modulation of inactivation correlates with the voltage-gated motion between the NH(2)-termini of alpha(1C)- and beta-subunits and targets the mechanism of slow voltage-dependent inactivation.
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