ArticleBMC microbiology2004
Towards the development of a DNA-sequence based approach to serotyping of Salmonella enterica.
Article in BMC microbiology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- Review
- Genetic diversity of the O antigens of Proteus species and the development of a suspension array for molecular serotyping.PloS one · 2017Article
- Distributions of Salmonella subtypes differ between two U.S. produce-growing regions.Applied and environmental microbiology · 2014Article
- Comparison of typing methods with a new procedure based on sequence characterization for Salmonella serovar prediction.Journal of clinical microbiology · 2013Article
- Rapid genoserotyping tool for classification of Salmonella serovars.Journal of clinical microbiology · 2011Article
- The analysis of groEL gene in Salmonella enterica subspecies enterica serovar Typhimurium isolated from avians by PCR-Restriction Fragment Length Polymorphism method.Veterinary research communications · 2011Article
- PCR-Restriction Fragment Length Polymorphism analysis of fljB gene in Salmonella enterica subspecies enterica serovar Typhimurium isolated from avians.Iranian journal of microbiology · 2010Article
- Characterization of 13 multi-drug resistant Salmonella serovars from different broiler chickens associated with those of human isolates.BMC microbiology · 2010Article
- Identification by PCR of non-typhoidal Salmonella enterica serovars associated with invasive infections among febrile patients in Mali.PLoS neglected tropical diseases · 2010Article
- The O28 Antigen Gene Clusters of Salmonella enterica subsp. enterica Serovar Dakar and Serovar Pomona Are Different.International journal of microbiology · 2010Article
- Comparison of classical serotyping and PremiTest assay for routine identification of common Salmonella enterica serovars.Journal of clinical microbiology · 2008Article
- Development of an oligonucleotide microarray method for Salmonella serotyping.Microbial biotechnology · 2008Article
- Multiplex PCR-based method for identification of common clinical serotypes of Salmonella enterica subsp. enterica.Journal of clinical microbiology · 2006Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe fliC and fljB genes in Salmonella code for the phase 1 (H1) and phase 2 (H2) flagellin respectively, the rfb cluster encodes the majority of enzymes for polysaccharide (O) antigen biosynthesis, together they determine the antigenic profile by which Salmonella are identified. Sequencing and characterisation of fliC was performed in the development of a molecular serotyping technique.
resultsFliC sequencing of 106 strains revealed two groups; the g-complex included those exhibiting "g" or "m,t" antigenic factors, and the non-g strains which formed a second more diverse group. Variation in fliC was characterised and sero-specific motifs identified. Furthermore, it was possible to identify differences in certain H antigens that are not detected by traditional serotyping. A rapid short sequencing assay was developed to target serotype-specific sequence motifs in fliC. The assay was evaluated for identification of H1 antigens with a panel of 55 strains.
conclusionFliC sequences were obtained for more than 100 strains comprising 29 different H1 alleles. Unique pyrosequencing profiles corresponding to the H1 component of the serotype were generated reproducibly for the 23 alleles represented in the evaluation panel. Short read sequence assays can now be used to identify fliC alleles in approximately 97% of the 50 medically most important Salmonella in England and Wales. Capability for high throughput testing and automation give these assays considerable advantages over traditional methods.
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