ArticleProceedings of the National Academy of Sciences of the United States of America2004
MED16 and MED23 of Mediator are coactivators of lipopolysaccharide- and heat-shock-induced transcriptional activators.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed, 77 citations in OpenAlex.
- Review
- Intersecting impact of CAG repeat and huntingtin knockout in stem cell-derived cortical neurons.Neurobiology of disease · 2025Article
- Review
- The CDK8 kinase module: A novel player in the transcription of translation initiation and ribosomal genes.Molecular biology of the cell · 2025Article
- Moesin contributes to heat shock gene response through direct binding to the Med15 subunit of the Mediator complex in the nucleus.Open biology · 2024Article
- Whole-Genome and Poly(A)+Transcriptome Analysis of theInternational journal of molecular sciences · 2024Article
- Infection by a helminth parasite is associated with changes in DNA methylation in the house sparrow.Ecology and evolution · 2022Article
- Connection of core and tail Mediator modules restrains transcription from TFIID-dependent promoters.PLoS genetics · 2021Article
- Loss of Function Genetic Screen Identifies ATM Kinase as a Positive Regulator of TLR3-Mediated NF-κB Activation.iScience · 2020Article
- Last-Generation Genome-Environment Associations Reveal the Genetic Basis of Heat Tolerance in Common Bean (Frontiers in genetics · 2019Article
- Mediator Is Essential for Small Nuclear and Nucleolar RNA Transcription in Yeast.Molecular and cellular biology · 2018Article
- ETV4 and AP1 Transcription Factors Form Multivalent Interactions with three Sites on the MED25 Activator-Interacting Domain.Journal of molecular biology · 2017Article
- The nematode homologue of Mediator complex subunit 28, F28F8.5, is a critical regulator ofPeerJ · 2017Article
- The Mediator Subunit MED16 Transduces NRF2-Activating Signals into Antioxidant Gene Expression.Molecular and cellular biology · 2016Article
- Recruitment of Mediator Complex by Cell Type and Stage-Specific Factors Required for Tissue-Specific TAF Dependent Gene Activation in an Adult Stem Cell Lineage.PLoS genetics · 2015Article
- ERK phosphorylation of MED14 in promoter complexes during mitogen-induced gene activation by Elk-1.Nucleic acids research · 2013Article
- A link between the cytoplasmic engulfment protein Elmo1 and the Mediator complex subunit Med31.Current biology : CB · 2013Article
- The Arabidopsis mediator complex subunit16 positively regulates salicylate-mediated systemic acquired resistance and jasmonate/ethylene-induced defense pathways.The Plant cell · 2012Article
- MED25 is a mediator component of HNF4α-driven transcription leading to insulin secretion in pancreatic beta-cells.PloS one · 2012Article
- The Mediator complex in plants: structure, phylogeny, and expression profiling of representative genes in a dicot (Arabidopsis) and a monocot (rice) during reproduction and abiotic stress.Plant physiology · 2011Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Transcriptional activators interact with diverse proteins and recruit transcriptional machinery to the activated promoter. Recruitment of the Mediator complex by transcriptional activators is usually the key step in transcriptional activation. However, it is unclear how Mediator recognizes different types of activator proteins. To systematically identify the subunits responsible for the signal- and activator-specific functions of Mediator in Drosophila melanogaster, each Mediator subunit was depleted by RNA interference, and its effect on transcriptional activation of endogenous as well as synthetic promoters was examined. The depletion of some Mediator gene products caused general transcriptional defects, whereas depletion of others caused defects specifically related to activation. In particular, MED16 and MED23 were required for lipopolysaccharide- and heat-shock-specific gene expression, respectively, and their activator-specific functions appeared to result from interaction with specific activators. The corequirement of MED16 for other forms of differentiation-inducing factor-induced transcription was confirmed by microarray analysis of differentiation-inducing factor (DIF)- and MED16-depleted cells individually. These results suggest that distinct Mediator subunits interact with specific activators to coordinate and transfer activator-specific signals to the transcriptional machinery.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.