ArticleJournal of clinical microbiology2004
Comparison of real-time PCR signal-amplified in situ hybridization and conventional PCR for detection and quantification of human papillomavirus in archival cervical cancer tissue.
Article in Journal of clinical microbiology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 84 citations in OpenAlex.
- Expression of AMHR2 and C-KIT in cervical lesions in Uyghur Women of Xinjiang, China.Medicine · 2018Trial
- The Predictive Impact of HPV Genotypes, Tumor Suppressors and Local Immune Response in the Regression of Cervical Intraepithelial Neoplasia 2-3: A Prospective Population-Based Cohort Study.International journal of molecular sciences · 2025Article
- Human papillomavirus type-specific distribution in cervical intraepithelial neoplasia and cancer in The Gambia prior to HPV immunization programme: a baseline for monitoring the quadrivalent vaccine.Infectious agents and cancer · 2024Article
- Genetic diversity, variation and recombination among the human papillomaviruses (HPVs) genomes isolated in China: a comparative genomic and phylogenetic analysis.Pathogens and global health · 2024Article
- Cytauxzoon felis in salivary glands of Amblyomma americanum.Ticks and tick-borne diseases · 2023Article
- Development of Quantitative Rapid Isothermal Amplification Assay forDiagnostics (Basel, Switzerland) · 2021Article
- Canine bocavirus-2 infection and its possible association with encephalopathy in domestic dogs.PloS one · 2021Article
- A fluorometric hybridization assay for detecting and genotyping high-risk human papillomavirus 16 and 18 in archival tissues of cervical specimens.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2020Article
- The Clinical Relevance of p16 and p53 Status in Patients with Squamous Cell Carcinoma of the Vulva.Journal of oncology · 2020Article
- Prevalence of human papillomavirus genotypes in cervical cancer in Maiduguri, Nigeria.The Pan African medical journal · 2019Article
- Elevated Expression of Ox2R in Cervical Cancers and Placentas of Uyghur Women in Xinjiang, ChinaAsian Pacific journal of cancer prevention : APJCP · 2016Article
- Low prevalence of HPV detection and genotyping in non-muscle invasive bladder cancer using single-step PCR followed by reverse line blot.World journal of urology · 2015Article
- Is real-time PCR the correct method to evaluate the incidence of human papillomavirus in prepuces of asymptomatic boys and men?World journal of urology · 2014Article
- Reduced expression of members of the mhc-i antigen processing machinery in ethnic Uighur women with cervical cancer in the Xinjiang region of China.Current oncology (Toronto, Ont.) · 2014Article
- Is human papillomavirus associated with prostate cancer survival?Disease markers · 2013Article
- Expression pattern of class I histone deacetylases in vulvar intraepithelial neoplasia and vulvar cancer: a tissue microarray study.BMC cancer · 2011Article
- Comparative analysis of four methods to extract DNA from paraffin-embedded tissues: effect on downstream molecular applications.BMC research notes · 2010Article
- Rapid, sensitive, type specific PCR detection of the E7 region of human papillomavirus type 16 and 18 from paraffin embedded sections of cervical carcinoma.Infectious agents and cancer · 2010Article
- Critical evaluation of HPV16 gene copy number quantification by SYBR green PCR.BMC biotechnology · 2008Article
- Evaluation of a commercialized in situ hybridization assay for detecting human papillomavirus DNA in tissue specimens from patients with cervical intraepithelial neoplasia and cervical carcinoma.Journal of clinical microbiology · 2008Article
Corrections and comments
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Authors and funding
9 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Archival paraffin-embedded tumor specimens offer a wealth of information for both cancer research and for routine clinical applications. However, the use of formalin-fixed, paraffin-embedded specimens for quantitative real-time PCR is not yet a standard diagnostic method in many laboratories, in particular for the quantification of human papillomavirus (HPV). Particularly high-risk HPV types are involved in almost 100% of the carcinogenesis of cervical cancer. We compared the diagnostic applicability and sensitivity of real-time PCR to that of chromogenic tyramide-signal-amplified in situ hybridization and conventional PCR for the detection of HPV from archival tissue in 164 cases of carcinoma in situ and cervical cancer. Furthermore, we examined whether the viral load of HPV is of prognostic relevance. Our findings indicate that patients in tumor stage I with a lower viral load of HPV type 16 (HPV16; up to 1,000 copies/ng of DNA) had a significantly better survival than HPV 16-negative patients (P = 0.037). We observed a greater sensitivity of both real-time PCR and conventional PCR for the detection of HPV16 and -18 compared to signal amplified in situ hybridization. We found a considerable concordance between HPV16 (kappa = 0.661) and HPV18 (kappa = 0.781) status as measured by real-time PCR and conventional PCR, indicating similar sensitivities. We recognized an inhibitory effect of formalin fixation and paraffin embedding on the evaluation of real-time PCR quantification.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.