ArticleJournal of clinical microbiology2004
High-throughput detection of pathogenic yeasts of the genus trichosporon.
Article in Journal of clinical microbiology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
48 citing papers in PubMed, 124 citations in OpenAlex.
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- High-throughput sequencing and characterization of potentially pathogenic fungi from the vaginal mycobiome of giant panda (Frontiers in microbiology · 2024Article
- Pericardial Effusion Due toPathogens (Basel, Switzerland) · 2022Article
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- Identification, genotyping, and pathogenicity of Trichosporon spp. Isolated from Giant pandas (Ailuropoda melanoleuca).BMC microbiology · 2019Article
- Invasive Infections Due toJournal of clinical microbiology · 2019Article
- Article
- Evaluating and Improving Vitek MS for Identification of Clinically Relevant Species of Trichosporon and the Closely Related Genera Cutaneotrichosporon and Apiotrichum.Journal of clinical microbiology · 2017Article
- xMAP Technology: Applications in Detection of Pathogens.Frontiers in microbiology · 2017Review
- Spatial and compositional variation in the fungal communities of organic and conventionally grown apple fruit at the consumer point-of-purchase.Horticulture research · 2016Article
- On and Under the Skin: Emerging Basidiomycetous Yeast Infections Caused by Trichosporon Species.PLoS pathogens · 2015Review
- LUMINEX®: a new technology for the simultaneous identification of five Entamoeba spp. commonly found in human stools.Parasites & vectors · 2013Article
- Invasive candidiasis in Pakistan: clinical characteristics, species distribution and antifungal susceptibility.Journal of medical microbiology · 2013Article
- Species identification of invasive yeasts including Candida in Pakistan: limitations of phenotypic methods.JPMA. The Journal of the Pakistan Medical Association · 2012Article
- Identification of novel hybrids between Cryptococcus neoformans var. grubii VNI and Cryptococcus gattii VGII.Mycopathologia · 2012Article
- Three-locus identification, genotyping, and antifungal susceptibilities of medically important Trichosporon species from China.Journal of clinical microbiology · 2011Article
- Current knowledge of Trichosporon spp. and Trichosporonosis.Clinical microbiology reviews · 2011Review
- Trichosporon inkin Esophagitis: An Uncommon Disease in a Patient with Pulmonary Cancer.Mycopathologia · 2011Article
- Diagnosis of invasive fungal infections by a real-time panfungal PCR assay in immunocompromised pediatric patients.Leukemia · 2010Article
- Molecular detection of harmful algal blooms (HABs) using locked nucleic acids and bead array technology.Limnology and oceanography, methods · 2010Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The need for a rapid and accurate method for the detection of fungal pathogens has become imperative as the incidence of fungal infections has increased dramatically. Herein, we tested the Luminex 100, a novel flow cytometer, for the detection of the medically important genus Trichosporon. This genus was selected as our proof-of-concept model due to the close phylogenetic relationship between the species. The method, which is based on a nucleotide hybridization assay, consists of a combination of different sets of fluorescent beads covalently bound to species-specific capture probes. Upon hybridization, the beads bearing the target amplicons are classified by their spectral addresses with a 635-nm laser. Quantitation of the hybridized biotinylated amplicon is based on fluorescence detection with a 532-nm laser. We tested in various multiplex formats 48 species-specific and group-specific capture probes designed in the D1/D2 region of ribosomal DNA, internal transcribed spacer regions, and intergenic spacer region. Species-specific biotinylated amplicons were generated with three sets of primers to yield fragments from the three regions. The assay was specific and fast, as it discriminated species differing by 1 nucleotide and required less than 50 min following amplification to process a 96-well plate. The sensitivity of the assay allowed the detection of 10(2) genome molecules in PCRs and 10(7) to 10(8) molecules of biotinylated amplification product. This technology provided a rapid means of detection of Trichosporon species with the flexibility to identify species in a multiplex format by combining different sets of beads.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.