ArticleJournal of clinical microbiology2004
Use of fluorescent amplified fragment length polymorphism for molecular epidemiology of leptospirosis in India.
Article in Journal of clinical microbiology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
14 citing papers in PubMed, 42 citations in OpenAlex.
- Leptospirosis in India: insights on circulating serovars, research lacunae and proposed strategies to control through one health approach.One health outlook · 2024Review
- Homologous microscopic agglutinating antibodies after natural infection with leptospires - results from a long term follow up of a cohort living in an endemic area.Pathogens and global health · 2017Article
- Profiling of Leptospira interrogans, L. santarosai, L. meyeri and L. borgpetersenii by SE-AFLP, PFGE and susceptibility testing--a continuous attempt at species and serovar differentiation.Emerging microbes & infections · 2016Article
- Use of a New High Resolution Melting Method for Genotyping Pathogenic Leptospira spp.PloS one · 2015Article
- Genotyping Tools forMycobacterial diseases : tuberculosis & leprosy · 2014Article
- Molecular epidemiology of Leptospira borgpetersenii serovar Arborea, Queensland, Australia, 1998-2005.The American journal of tropical medicine and hygiene · 2010Article
- Application and validation of PFGE for serovar identification of Leptospira clinical isolates.PLoS neglected tropical diseases · 2010Article
- Article
- Leptospirosis in the Asia Pacific region.BMC infectious diseases · 2009Review
- Distribution of the leptospiral immunoglobulin-like (lig) genes in pathogenic Leptospira species and application of ligB to typing leptospiral isolates.Journal of medical microbiology · 2009Article
- Leptospirosis: The "mysterious" mimic.Journal of emergencies, trauma, and shock · 2008Article
- Utility of phylogenetic studies in the identification of Leptospira strains.Epidemiology and infection · 2007Article
- Multilocus sequence typing method for identification and genotypic classification of pathogenic Leptospira species.Annals of clinical microbiology and antimicrobials · 2006Article
- Development of a Multiple-Locus Variable Number of Tandem Repeat Analysis (MLVA) for Leptospira interrogans and its application to Leptospira interrogans serovar Australis isolates from Far North Queensland, Australia.Annals of clinical microbiology and antimicrobials · 2005Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Nineteen isolates of leptospires recovered from patients during three epidemics that occurred at different places and different times in the Andaman Islands and eight isolates from sporadic cases were characterized using serological and molecular genetic techniques. Group sera and monoclonal antibodies were used for antigenic characterization, whereas fluorescent amplified fragment length polymorphism (FAFLP) was used for genotyping. Of the 27 isolates, 19 were identified as belonging to serogroup Grippotyphosa, 3 belonged to serogroup Australis, 2 belonged to serogroup Icterohaemorrhagiae, and 1 each belonged to serogroups Hebdomadis, Canicola, and Sejroe. Analysis of FAFLP data grouped these 27 isolates into two main clusters of genotypes. One of the clusters, populated by 19 isolates, included 16 outbreak isolates. Seven of these 19 isolates belonged to serovar Ratnapura, 10 belonged to serovar Valbuzzi, and 1 each belonged to serovar Grippotyphosa and serovar Saxkoebing. Of the 27 patients from whom isolates were obtained, 9 had severe illness, and 6 of these 9 patients had pulmonary involvement, 1 had pulmonary and hepatorenal involvement, and the remaining 2 had hepatorenal involvement alone. Two patients out of the nine severe cases died subsequently. The isolates from sporadic cases showed great genetic diversity and were also diverse antigenically. Perhaps the strains belonging to a dominant genotype (the outbreak-associated cluster) possessed epidemic potential and higher virulence with a greater predilection to cause pulmonary complications than strains belonging to other genetic backgrounds.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.