ArticleJournal of clinical microbiology2004
Use of 16S ribosomal DNA PCR and denaturing gradient gel electrophoresis for analysis of the microfloras of healing and nonhealing chronic venous leg ulcers.
Article in Journal of clinical microbiology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers, 1 of them a synthesis that pooled it.
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Who cites it
48 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Topical antimicrobial agents for treating foot ulcers in people with diabetes.The Cochrane database of systematic reviews · 2017Pooled it
- Investigation of bacterial agents in yaws-like ulcers in children and their environment in Cameroon: An experience from the Akonolinga Health District.PLoS neglected tropical diseases · 2026Article
- Wound Healing Property of a Novel Thermo-Reversible Wound Gel With Lasting Antimicrobial and Antibiofilm Activity.International wound journal · 2026Article
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- Biofilm-mediated infections by multidrug-resistant microbes: a comprehensive exploration and forward perspectives.Archives of microbiology · 2024Review
- Silver Nanoparticles Phytofabricated throughAntibiotics (Basel, Switzerland) · 2023Article
- Biofilm-Associated Infections in Chronic Wounds and Their Management.Advances in experimental medicine and biology · 2023Article
- Metagenomic features of bioburden serve as outcome indicators in combat extremity wounds.Scientific reports · 2022Article
- Biofilm Survival Strategies in Chronic Wounds.Microorganisms · 2022Review
- Advances in the Sensing and Treatment of Wound Biofilms.Angewandte Chemie (International ed. in English) · 2022Review
- Advances in the Sensing and Treatment of Wound Biofilms.Angewandte Chemie (Weinheim an der Bergstrasse, Germany) · 2022Review
- [Research advances on the formation mechanism and diagnosis of bacteria biofilms in chronic wounds].Zhonghua shao shang za zhi = Zhonghua shaoshang zazhi = Chinese journal of burns · 2021Article
- Effect of collagen and EPS components on the viscoelasticity ofSoft matter · 2021Article
- Real time monitoring of biofilm formation on coated medical devices for the reduction and interception of bacterial infections.Biomaterials science · 2020Article
- Association between baseline abundance of Peptoniphilus, a Gram-positive anaerobic coccus, and wound healing outcomes of DFUs.PloS one · 2020Article
- The diagnosis of infection in chronic leg ulcers: A narrative review on clinical practice.International wound journal · 2019Review
- The correlation of crystalline and elemental composition of urinary stones with a history of bacterial infections: TXRF, XRPD and PCR-DGGE studies.European biophysics journal : EBJ · 2019Article
- Molecular Techniques Complement Culture-Based Assessment of Bacteria Composition in Mixed Biofilms of Urinary Tract Catheter-Related Samples.Frontiers in microbiology · 2019Article
- High‑throughput sequencing analyses of oral microbial diversity in healthy people and patients with dental caries and periodontal disease.Molecular medicine reports · 2017Article
- Agarose bioplastic-based drug delivery system for surgical and wound dressings.Engineering in life sciences · 2017Article
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The bacterial microfloras of 8 healing and 10 nonhealing chronic venous leg ulcers were compared by using a combination of cultural analysis and denaturing gradient gel electrophoresis (DGGE) of PCR-amplified 16S rRNA gene products. Cultural analysis of the microflora revealed that the majority of both wound types carried the aerobes Staphylococcus and Pseudomonas spp. (89 and 80%, respectively). Sequencing of 16S ribosomal DNAs selected on the basis of DGGE profiling allowed the identification of strains not detected by cultural means. Of considerable interest was the finding that more than 40% of the sequences represented organisms not cultured from the wound from which they were amplified. DGGE profiles also revealed that all of the wounds possessed one apparently common band, identified by sequencing as Pseudomonas sp. The intensity of this PCR signal suggested that the bacterial load of nonhealing wounds was much higher for pseudomonads compared to healing wounds and that it may have been significantly underestimated by cultural analysis. Hence, the present study shows that DGGE could give valuable additional information about chronic wound microflora that is not apparent from cultural analysis alone.
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