ArticleJournal of clinical microbiology2004
Rapid differentiation of Aspergillus species from other medically important opportunistic molds and yeasts by PCR-enzyme immunoassay.
Article in Journal of clinical microbiology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 74 citations in OpenAlex.
- Whole genome sequence characterization of Aspergillus terreus ATCC 20541 and genome comparison of the fungi A. terreus.Scientific reports · 2023Article
- Evaluation of molecular identification ofSaudi journal of biological sciences · 2020Article
- LC-MS/MS Tandem Mass Spectrometry for Analysis of Phenolic Compounds and Pentacyclic Triterpenes in Antifungal Extracts of Terminalia brownii (Fresen).Antibiotics (Basel, Switzerland) · 2017Article
- Aspergillus galactomannan detection in comparison to a real-time PCR assay in serum samples from a high-risk group of patients.Central-European journal of immunology · 2015Article
- Development of RFLP-PCR method for the identification of medically important Aspergillus species using single restriction enzyme MwoI.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2014Article
- Detection of invasive aspergillosis in bone marrow transplant recipients using real-time PCR.Journal of global infectious diseases · 2013Article
- Clinical and laboratory signs as dengue markers during an outbreak in Rio de Janeiro.Infection · 2008Article
- Reverse line blot hybridization assay for identification of medically important fungi from culture and clinical specimens.Journal of clinical microbiology · 2007Article
- Development of novel real-time PCR assays for detection and differentiation of eleven medically important Aspergillus and Candida species in clinical specimens.Journal of clinical microbiology · 2007Article
- Simultaneous detection and identification of Candida, Aspergillus, and Cryptococcus species by reverse line blot hybridization.Journal of clinical microbiology · 2006Article
- Identification of medically important molds by an oligonucleotide array.Journal of clinical microbiology · 2005Article
- Use of a suspension array for rapid identification of the varieties and genotypes of the Cryptococcus neoformans species complex.Journal of clinical microbiology · 2005Article
- Assessment of ribosomal large-subunit D1-D2, internal transcribed spacer 1, and internal transcribed spacer 2 regions as targets for molecular identification of medically important Aspergillus species.Journal of clinical microbiology · 2005Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We developed a PCR-based assay to differentiate medically important species of Aspergillus from one another and from other opportunistic molds and yeasts by employing universal, fungus-specific primers and DNA probes in an enzyme immunoassay format (PCR-EIA). Oligonucleotide probes, directed to the internal transcribed spacer 2 region of ribosomal DNA from Aspergillus flavus, Aspergillus fumigatus, Aspergillus nidulans, Aspergillus niger, Aspergillus terreus, Aspergillus ustus, and Aspergillus versicolor, differentiated 41 isolates (3 to 9 each of the respective species; P < 0.001) in a PCR-EIA detection matrix and gave no false-positive reactions with 33 species of Acremonium, Exophiala, Candida, Fusarium, Mucor, Paecilomyces, Penicillium, Rhizopus, Scedosporium, Sporothrix, or other aspergilli tested. A single DNA probe to detect all seven of the most medically important Aspergillus species (A. flavus, A. fumigatus, A. nidulans, A. niger, A. terreus, A. ustus, and A. versicolor) was also designed. Identification of Aspergillus species was accomplished within a single day by the PCR-EIA, and as little as 0.5 pg of fungal DNA could be detected by this system. In addition, fungal DNA extracted from tissues of experimentally infected rabbits was successfully amplified and identified using the PCR-EIA system. This method is simple, rapid, and sensitive for the identification of medically important Aspergillus species and for their differentiation from other opportunistic fungi.
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