ArticleApplied and environmental microbiology2004
VdNEP, an elicitor from Verticillium dahliae, induces cotton plant wilting.
Article in Applied and environmental microbiology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 55 papers.
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Who cites it
55 citing papers in PubMed, 129 citations in OpenAlex.
- Moderately ectopic expressing NLP27 in potato confers high disease resistance without yield penalty.Plant cell reports · 2026Article
- Fungal foe: exploring cotton's physiological responses to Verticillium wilt.Frontiers in plant science · 2026Review
- Interaction of an Oomycete Nep1-like Cytolysin with Natural and Plant Cell-Mimicking Membranes.The Journal of membrane biology · 2025Article
- Polyethyleneimine-coated MXene quantum dots improve cotton tolerance to Verticillium dahliae by maintaining ROS homeostasis.Nature communications · 2023Article
- Protein Kinase C Is Involved in Vegetative Development, Stress Response and Pathogenicity inInternational journal of molecular sciences · 2023Article
- Dirigent gene editing of gossypol enantiomers for toxicity-depleted cotton seeds.Nature plants · 2023Article
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- Investigating Verticillium wilt occurrence in cotton and its risk management by the direct return of cotton plants infected withFrontiers in plant science · 2023Article
- Interactions betweenFrontiers in plant science · 2023Review
- Two Metalloproteases VdM35-1 and VdASPF2 from Verticillium dahliae Are Required for Fungal Pathogenicity, Stress Adaptation, and Activating Immune Response of Host.Microbiology spectrum · 2022Article
- The secretome of Verticillium dahliae in collusion with plant defence responses modulates Verticillium wilt symptoms.Biological reviews of the Cambridge Philosophical Society · 2022Review
- Identification and Functional Analysis of a Novel Hydrophobic Protein VdHP1 from Verticillium dahliae.Microbiology spectrum · 2022Article
- Integrative transcriptomic and gene co-expression network analysis of host responses upon Verticillium dahliae infection in Gossypium hirsutum.Scientific reports · 2021Article
- Remote homology clustering identifies lowly conserved families of effector proteins in plant-pathogenic fungi.Microbial genomics · 2021Article
- Verticillium dahliae secreted protein Vd424Y is required for full virulence, targets the nucleus of plant cells, and induces cell death.Molecular plant pathology · 2021Article
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- Diversity and Biocontrol Potential of Cultivable Endophytic Bacteria Associated with Halophytes from the West Aral Sea Basin.Microorganisms · 2021Article
- The Role of a New Compound Micronutrient Multifunctional Fertilizer againstPathogens (Basel, Switzerland) · 2021Article
- Genome-Wide Analysis of the Cytochrome P450 Gene Family Involved in Salt Tolerance inFrontiers in plant science · 2021Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Verticillium wilt is a vascular disease of cotton. The causal fungus, Verticillium dahliae, secretes elicitors in culture. We have generated approximately 1,000 5'-terminal expressed sequence tags (ESTs) from a cultured mycelium of V. dahliae. A number of ESTs were found to encode proteins harboring putative signal peptides for secretion, and their cDNAs were isolated. Heterologous expression led to the identification of a protein with elicitor activities. This protein, named V. dahliae necrosis- and ethylene-inducing protein (VdNEP), is composed of 233 amino acids and has high sequence identities with fungal necrosis- and ethylene-inducing proteins. Infiltration of the bacterially expressed His-VdNEP into Nicotiana benthamiana leaves resulted in necrotic lesion formation. In Arabidopsis thaliana, the fusion protein also triggered production of reactive oxygen species and induced the expression of PR genes. When added into suspension cultured cells of cotton (Gossypium arboreum), the fusion protein elicited the biosynthesis of gossypol and related sesquiterpene phytoalexins at low concentrations, and it induced cell death at higher concentrations. On cotton cotyledons and leaves, His-VdNEP induced dehydration and wilting, similar to symptoms caused by a crude preparation of V. dahliae elicitors. Northern blotting showed a low level of VdNEP expression in the mycelium during culture. These data suggest that VdNEP is a wilt-inducing factor and that it participates in cotton-V. dahliae interactions.
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