ArticleApplied and environmental microbiology2004
Differential gene expression to investigate the effect of (5Z)-4-bromo- 5-(bromomethylene)-3-butyl-2(5H)-furanone on Bacillus subtilis.
Article in Applied and environmental microbiology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 55 citations in OpenAlex.
- The Novel Chiral 2(5Molecules (Basel, Switzerland) · 2023Article
- Antimicrobial and Biofilm-Preventing Activity of l-Borneol Possessing 2(5H)-Furanone Derivative F131 against S. aureus—C. albicans Mixed CulturesPathogens (Basel, Switzerland) · 2022Article
- Natural Strategies as Potential Weapons against Bacterial Biofilms.Life (Basel, Switzerland) · 2022Review
- Persister control by leveraging dormancy associated reduction of antibiotic efflux.PLoS pathogens · 2021Article
- Unraveling the Molecular Mechanism of Selective Antimicrobial Activity of 2(5International journal of molecular sciences · 2019Article
- Streptomyces sp. AT37 isolated from a Saharan soil produces a furanone derivative active against multidrug-resistant Staphylococcus aureus.World journal of microbiology & biotechnology · 2017Article
- Antimicrobial Effects of Sulfonyl Derivative of 2(5Frontiers in microbiology · 2017Article
- 5-Episinuleptolide Decreases the Expression of the Extracellular Matrix in Early Biofilm Formation of Multi-Drug Resistant Acinetobacter baumannii.Marine drugs · 2016Article
- Global transcriptional analysis of Burkholderia pseudomallei high and low biofilm producers reveals insights into biofilm production and virulence.BMC genomics · 2015Article
- Inhibition of biofilm formation in Bacillus subtilis by new halogenated furanones.The Journal of antibiotics · 2015Article
- Differential Gene Expression to Investigate the Effects of Low-level Electrochemical Currents on Bacillus subtilis.AMB Express · 2011Article
- Covalent immobilization of antibacterial furanones via photochemical activation of perfluorophenylazide.Langmuir : the ACS journal of surfaces and colloids · 2009Article
- Brominated furanones inhibit biofilm formation by Salmonella enterica serovar Typhimurium.Applied and environmental microbiology · 2008Article
- Cross-kingdom signalling: exploitation of bacterial quorum sensing molecules by the green seaweed Ulva.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2007Review
- Differential gene expression for investigation of Escherichia coli biofilm inhibition by plant extract ursolic acid.Applied and environmental microbiology · 2005Article
- Article
- Time-Dependent Toxicities of Quorum Sensing Inhibitors toDose-response : a publication of International Hormesis SocietyArticle
Corrections and comments
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Authors and funding
8 authors at 6 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
(5Z)-4-Bromo-5-(bromomethylene)-3-butyl-2(5H)-furanone (furanone) from the red marine alga Delisea pulchra was found previously to inhibit the growth, swarming, and biofilm formation of gram-positive bacteria. Using the gram-positive bacterium Bacillus subtilis as a test organism, we observed cell killing by 20 microg of furanone per ml, while 5 microg of furanone per ml inhibited growth approximately twofold without killing the cells. To discover the mechanism of this inhibition on a genetic level and to investigate furanone as a novel antibiotic, full-genome DNA microarrays were used to analyze the gene expression profiles of B. subtilis grown with and without 5 microg of furanone per ml. This agent induced 92 genes more than fivefold (P < 0.05) and repressed 15 genes more than fivefold (P < 0.05). The induced genes include genes involved in stress responses (such as the class III heat shock genes clpC, clpE, and ctsR and the class I heat shock genes groES, but no class II or IV heat shock genes), fatty acid biosynthesis, lichenan degradation, transport, and metabolism, as well as 59 genes with unknown functions. The microarray results for four genes were confirmed by RNA dot blotting. Mutation of a stress response gene, clpC, caused B. subtilis to be much more sensitive to 5 microg of furanone per ml (there was no growth in 8 h, while the wild-type strain grew to the stationary phase in 8 h) and confirmed the importance of the induction of this gene as identified by the microarray analysis.
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