ArticleApplied and environmental microbiology2004
Expression and immunogenicity of a recombinant diphtheria toxin fragment A in Streptococcus gordonii.
Article in Applied and environmental microbiology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
6 citing papers in PubMed.
- Recent development of oral vaccines (Review).Experimental and therapeutic medicine · 2024Review
- Exploring Host-Commensal Interactions in the Respiratory Tract.Frontiers in immunology · 2017Review
- Expression and purification of truncated diphtheria toxin, DT386, inResearch in pharmaceutical sciences · 2016Article
- Expression of a functional single-chain variable-fragment antibody against complement receptor 1 in Streptococcus gordonii.Clinical and vaccine immunology : CVI · 2008Article
- Construction and characterization of single-chain variable fragment antibodies directed against the Bordetella pertussis surface adhesins filamentous hemagglutinin and pertactin.Infection and immunity · 2007Article
- Role of D-alanylation of Streptococcus gordonii lipoteichoic acid in innate and adaptive immunity.Infection and immunity · 2007Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A nontoxic mutant diphtheria toxin fragment A (DTA) was genetically fused in single, double, or triple copy to the major surface protein antigen P1 (SpaP) and surface expressed in Streptococcus gordonii DL-1. The expression was verified by Western immunoblotting. Mouse antisera raised against the recombinant S. gordonii recognized the native diphtheria toxinm suggesting the recombinant DTA was immunogenic. When given intranasally to mice with cholera toxin subunit B as the adjuvant, the recombinant S. gordonii expressing double copies of DTA (SpaP-DTA(2)) induced a mucosal immunoglobulin A response and a weak systemic immunoglobulin G response. S. gordonii SpaP-DTA(2) was able to orally colonize BALB/c mice for a 15-week period and elicited a mucosal response, but a serum immunoglobulin G response was not apparent. The antisera failed to neutralize diphtheria toxin cytotoxicity in a Vero cell assay.
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Registered trials
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