ArticleMolecular biology reports2004
Expression of a transgene encoded on a non-viral episomal vector is not subject to epigenetic silencing by cytosine methylation.
Article in Molecular biology reports, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 49 citations in OpenAlex.
- Improving Wound Healing with DICER1-Modified Keratinocytes.ACS omega · 2025Article
- Successful large gene augmentation of USH2A with non-viral episomal vectors.Molecular therapy : the journal of the American Society of Gene Therapy · 2023Article
- Shortened nuclear matrix attachment regions are sufficient for replication and maintenance of episomes in mammalian cells.Molecular biology of the cell · 2019Article
- Episomal minicircles persist in periods of transcriptional inactivity and can be transmitted through somatic cell nuclear transfer into bovine embryos.Molecular biology reports · 2019Article
- A chimeric HS4 insulator-scaffold attachment region enhances transgene expression in transfected Chinese hamster ovary cells.FEBS open bio · 2017Article
- Safe engineering of CAR T cells for adoptive cell therapy of cancer using long-term episomal gene transfer.EMBO molecular medicine · 2016Article
- Impact of Different Promoters on Episomal Vectors Harbouring Characteristic Motifs of Matrix Attachment Regions.Scientific reports · 2016Article
- Treatment of phenylketonuria using minicircle-based naked-DNA gene transfer to murine liver.Hepatology (Baltimore, Md.) · 2014Article
- Genetic modification of dividing cells using episomally maintained S/MAR DNA vectors.Molecular therapy. Nucleic acids · 2013Article
- Genetic modification of cancer cells using non-viral, episomal S/MAR vectors for in vivo tumour modelling.PloS one · 2012Article
- Non-viral S/MAR vectors replicate episomally in vivo when provided with a selective advantage.Gene therapy · 2011Article
- LDLR-Gene therapy for familial hypercholesterolaemia: problems, progress, and perspectives.International archives of medicine · 2010Article
- Stable S/MAR-based episomal vectors are regulated at the chromatin level.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2010Article
- Nanoparticles for retinal gene therapy.Progress in retinal and eye research · 2010Review
- pEPito: a significantly improved non-viral episomal expression vector for mammalian cells.BMC biotechnology · 2010Article
- Integration-deficient lentiviral vectors: a slow coming of age.Molecular therapy : the journal of the American Society of Gene Therapy · 2009Review
- Intrathecal injection of naked plasmid DNA provides long-term expression of secreted proteins.Molecular therapy : the journal of the American Society of Gene Therapy · 2009Article
- Persistent episomal transgene expression in liver following delivery of a scaffold/matrix attachment region containing non-viral vector.Gene therapy · 2008Article
- Long-term suppression of hepatitis B virus replication by short hairpin RNA expression using the scaffold/matrix attachment region-based replicating vector system pEPI-1.Antimicrobial agents and chemotherapy · 2008Article
- Establishment and mitotic stability of an extra-chromosomal mammalian replicon.BMC cell biology · 2007Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Currently available vectors for mammalian cells suffer from a number of limitations which make them only partially useful for genetic modification of eukaryotic cells and organisms and for gene therapy. While integration of a vector can lead to unpredictable interactions with the host genome and silencing of the integrated transgene, most non-integrating vectors mediate only transient expression of a transgene. All available vector types can lead to transformation of the recipient cell and many of them can cause serious immunological side effects in the organism. The ideal vector has to be free of these side effects and should allow long-term expression of a transgene in the absence of selection. In this report we describe a novel non-viral episomal expression system fulfilling these criteria. The gene encoding the truncated rat NGF-receptor gene under the control of the CMV-promoter was inserted into a vector construct containing a scaffold/matrix attached region (S/MAR). This vector was then transfected into CHO cells and human HaCat cells. We show that this vector replicates episomally in these cells and is mitotically stable in the abscence of selection over more than 100 generations. Moreover, we provide the first experimental data that the CMV-promoter in an episome is not subject to silencing by cytosine methylation, thus allowing long-term expression of the transgene in the absence of selection.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.