ArticleProceedings of the National Academy of Sciences of the United States of America2004
Tetrodotoxin-sensitive Na+ channels and muscarinic and purinergic receptors identified in human erythroid progenitor cells and red blood cell ghosts.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 50 citations in OpenAlex.
- Sodium channels in non-excitable cells: powerful actions and therapeutic targets beyond Hodgkin and Huxley.Trends in cell biology · 2025Review
- Non-canonical endogenous expression of voltage-gated sodium channel NaThe Journal of physiology · 2022Article
- Article
- Involvement of P2 receptors in hematopoiesis and hematopoietic disorders, and as pharmacological targets.Purinergic signalling · 2020Review
- ADP exerts P2YJournal of cell communication and signaling · 2020Article
- No transcriptional evidence for active NaChannels (Austin, Tex.) · 2019Article
- Purinergic control of red blood cell metabolism: novel strategies to improve red cell storage quality.Blood transfusion = Trasfusione del sangue · 2017Review
- Effects of Hypoxia on Erythrocyte Membrane Properties-Implications for Intravascular Hemolysis and Purinergic Control of Blood Flow.Frontiers in physiology · 2017Review
- Blood cells: an historical account of the roles of purinergic signalling.Purinergic signalling · 2015Review
- Piezo1 regulates mechanotransductive release of ATP from human RBCs.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Identification of signalling cascades involved in red blood cell shrinkage and vesiculation.Bioscience reports · 2015Article
- P2X and P2Y receptor signaling in red blood cells.Frontiers in molecular biosciences · 2015Review
- The Na(V)1.7 sodium channel: from molecule to man.Nature reviews. Neuroscience · 2013Review
- Nav1.7 is the predominant sodium channel in rodent olfactory sensory neurons.Molecular pain · 2011Article
- Alpha-hemolysin from Escherichia coli uses endogenous amplification through P2X receptor activation to induce hemolysis.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- Reduced DIDS-sensitive chloride conductance in Ae1-/- mouse erythrocytes.Blood cells, molecules & diseasesArticle
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
This article concerns the identification of different types of voltage-gated Na(+) channels and of muscarinic and purinergic receptors that are expressed in human erythroid precursor cells and red cell ghosts. We analyzed, by RT-PCR, RNA that was extracted from purified and synchronously growing human erythroid progenitor cells, differentiating from erythroblasts to reticulocytes in 7 to 14 days. These extracts were free of white cell and platelet contamination. Two types of voltage-gated, tetrodotoxin-sensitive Na(+) channels were found. These were Na(v)1.4 and Na(v)1.7, the former known to be present in skeletal muscle and the latter in peripheral nerve. By using a pan Na(+) channel antibody and Western blotting, an immunoreactive channel was detected in ghosts of human red blood cells, consistent with the expression of these two channels. The transcripts for four of the five known subtypes of muscarinic receptors were also identified, including subtypes M2, M3, M4, and M5, whereas subtype M1 was not found. Expression was also detected for the purinergic type receptors P2X(1), P2X(4), P2X(7), and P2Y(1) whereas types P2Y(2), P2Y(4), and P2Y(6) were not found. We also searched for but did not find transcripts for hBNP-1, a type 1b human brain sodium phosphate cotransporter, and cystic fibrosis transmembrane conductance regulator (CFTR). Implications regarding the presence of these different types of channels and receptors in human red blood cells and their functional significance are discussed.
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