ArticleMolecular biology of the cell2004
Localization of RNAs to the mitochondrial cloud in Xenopus oocytes through entrapment and association with endoplasmic reticulum.
Article in Molecular biology of the cell, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 64 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
64 citing papers in PubMed, 154 citations in OpenAlex.
- Decoding the complex journeys of RNAs along neurons.Nucleic acids research · 2025Article
- Statistical analysis supports pervasive RNA subcellular localization and alternative 3' UTR regulation.eLife · 2024Article
- Germ plasm dynamics during oogenesis and early embryonic development in Xenopus and zebrafish.Molecular reproduction and development · 2024Review
- Mechanistic insights into the basis of widespread RNA localization.Nature cell biology · 2024Review
- Oocyte Health and Quality: Implication of Mitochondria-related Organelle Interactions.Results and problems in cell differentiation · 2024Review
- The dynamic world of RNA: beyond translation to subcellular localization and function.Frontiers in genetics · 2024Article
- Friction forces determine cytoplasmic reorganization and shape changes of ascidian oocytes upon fertilization.Nature physics · 2024Article
- Solubility phase transition of maternal RNAs during vertebrate oocyte-to-embryo transition.Developmental cell · 2023Article
- RNA localization during early development of the axolotl.Frontiers in cell and developmental biology · 2023Article
- Regulation of RNA localization during oocyte maturation by dynamic RNA-ER association and remodeling of the ER.Cell reports · 2022Article
- Hitchhiking Across Kingdoms: Cotransport of Cargos in Fungal, Animal, and Plant Cells.Annual review of cell and developmental biology · 2022Review
- A proteomics approach identifies novel resident zebrafish Balbiani body proteins Cirbpa and Cirbpb.Developmental biology · 2022Article
- Linking transport and translation of mRNAs with endosomes and mitochondria.EMBO reports · 2021Review
- Spatio-temporal mRNA tracking in the early zebrafish embryo.Nature communications · 2021Article
- Mitochondria Lead the Way: Mitochondrial Dynamics and Function in Cellular Movements in Development and Disease.Frontiers in cell and developmental biology · 2021Review
- Exploring the Diverse Functional and Regulatory Consequences of Alternative Splicing in Development and Disease.Frontiers in genetics · 2021Review
- Phase Separation in Germ Cells and Development.Developmental cell · 2020Review
- Molecular mechanisms behind mRNA localization in axons.Open biology · 2020Review
- Mechanisms and consequences of subcellular RNA localization across diverse cell types.Traffic (Copenhagen, Denmark) · 2020Review
- mRNA Localization in Plant Cells.Plant physiology · 2020Review
4 more citing papers are in PubMed but not listed here.
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
Abstract
The germ cell lineage in Xenopus is specified by the inheritance of germ plasm, which originates within a distinct "mitochondrial cloud" (MC) in previtellogenic oocytes. Germ plasm contains localized RNAs implicated in germ cell development, including Xcat2 and Xdazl. To understand the mechanism of the early pathway through which RNAs localize to the MC, we applied live confocal imaging and photobleaching analysis to oocytes microinjected with fluorescent Xcat2 and Xdazl RNA constructs. These RNAs dispersed evenly throughout the cytoplasm through diffusion and then became progressively immobilized and formed aggregates in the MC. Entrapment in the MC was not prevented by microtubule disruption and did not require localization to germinal granules. Immobilized RNA constructs codistributed and showed coordinated movement with densely packed endoplasmic reticulum (ER) concentrated in the MC, as revealed with Dil16(3) labeling and immunofluorescence analysis. Vg1RBP/Vera protein, which has been implicated in linking late pathway RNAs to vegetal ER, was shown to bind specifically both wild-type Xcat2 3' untranslated region and localization-defective constructs. We found endogenous Vg1RBP/Vera and Vg1RBP/Vera-green fluorescent protein to be largely excluded from the MC but subsequently to codistribute with Xcat2 and ER at the vegetal cortex. We conclude that germ line RNAs localize into the MC through a diffusion/entrapment mechanism involving Vg1RBP/Vera-independent association with ER.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.