ArticleJournal of bacteriology2004
Molecular analysis of cytolysin A (ClyA) in pathogenic Escherichia coli strains.
Article in Journal of bacteriology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
37 citing papers in PubMed, 80 citations in OpenAlex.
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- Widespread Multidrug Resistance and Virulence Determinants inAntibiotics (Basel, Switzerland) · 2026Article
- RTX-family toxin EhxA drives morphological remodeling and thrombogenesis in RBCs during enterohemorrhagicScience advances · 2026Article
- Review
- Cytolysin A is an intracellularly induced and secreted cytotoxin of typhoidal Salmonella.Nature communications · 2024Article
- Genomic Characterization of International High-Risk Clone ST410Antibiotics (Basel, Switzerland) · 2022Article
- Characterization of Escherichia coli isolated from urinary tract infection and association between virulence expression and antimicrobial susceptibility.BMC microbiology · 2022Article
- Bacterial pore-forming toxins.Microbiology (Reading, England) · 2022Article
- Review
- Comparative Pathogenomics ofInfection and immunity · 2021Article
- Pore Forming Protein Induced Biomembrane Reorganization and Dynamics: A Focused Review.Frontiers in molecular biosciences · 2021Review
- Sequential CRISPR-Based Screens Identify LITAF and CDIP1 as the Bacillus cereus Hemolysin BL Toxin Host Receptors.Cell host & microbe · 2020Article
- Laboratory Diagnosis of Paratyphoid Fever: Opportunity of Surface Plasmon Resonance.Diagnostics (Basel, Switzerland) · 2020Review
- Evaluation of a Rapid Point-of-Care Multiplex Immunochromatographic Assay for the Diagnosis of Enteric Fever.mSphere · 2020Article
- Plasma Immunoglobulin A Responses Against 2 Salmonella Typhi Antigens Identify Patients With Typhoid Fever.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2019Article
- Designing novel construction for cell surface display of protein E on Escherichia coli using non-classical pathway based on Lpp-OmpA.AMB Express · 2017Article
- Soluble Oligomers of the Pore-forming Toxin Cytolysin A from Escherichia coli Are Off-pathway Products of Pore Assembly.The Journal of biological chemistry · 2016Article
- Assembling the puzzle: Oligomerization of α-pore forming proteins in membranes.Biochimica et biophysica acta · 2016Review
- Detection of Typhoidal and Paratyphoidal Salmonella in Blood by Real-time Polymerase Chain Reaction.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2015Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cytolysin A (ClyA) of Escherichia coli is a pore-forming hemolytic protein encoded by the clyA (hlyE, sheA) gene that was first identified in E. coli K-12. In this study we examined various clinical E. coli isolates with regard to the presence and integrity of clyA. PCR and DNA sequence analyses demonstrated that 19 of 23 tested Shiga toxin-producing E. coli (STEC) strains, all 7 tested enteroinvasive E. coli (EIEC) strains, 6 of 8 enteroaggregative E. coli (EAEC) strains, and 4 of 7 tested enterotoxigenic E. coli (ETEC) strains possess a complete clyA gene. The remaining STEC, EAEC, and ETEC strains and 9 of the 17 tested enteropathogenic E. coli (EPEC) strains were shown to harbor mutant clyA derivatives containing 1-bp frameshift mutations that cause premature termination of the coding sequence. The other eight EPEC strains and all tested uropathogenic and new-born meningitis-associated E. coli strains (n = 14 and 3, respectively) carried only nonfunctional clyA fragments due to the deletion of two sequences of 493 bp and 204 or 217 bp at the clyA locus. Expression of clyA from clinical E. coli isolates proved to be positively controlled by the transcriptional regulator SlyA. Several tested E. coli strains harboring a functional clyA gene produced basal amounts of ClyA when grown under standard laboratory conditions, but most of them showed a clyA-dependent hemolytic phenotype only when SlyA was overexpressed. The presented data indicate that cytolysin A can play a role only for some of the pathogenic E. coli strains.
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