ArticleJournal of bacteriology2004
The Escherichia coli GTPase CgtAE cofractionates with the 50S ribosomal subunit and interacts with SpoT, a ppGpp synthetase/hydrolase.
Article in Journal of bacteriology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 69 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
69 citing papers in PubMed, 153 citations in OpenAlex.
- Capturing ribosomal structures in cellular extracts with cryoPRISM: A purification-free cryoEM approach reveals novel structural states.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- The role of the essential GTPase ObgE in regulating lipopolysaccharide synthesis in Escherichia coli.Nature communications · 2024Article
- YbiB: a novel interactor of the GTPase ObgE.Nucleic acids research · 2023Article
- New perspectives on the molecular mechanisms of stress signalling by the nucleotide guanosine tetraphosphate (ppGpp), an emerging regulator of photosynthesis in plants and algae.The New phytologist · 2023Review
- The structure-function analysis of Obg-like GTPase proteins along the evolutionary tree from bacteria to humans.Genes to cells : devoted to molecular & cellular mechanisms · 2022Review
- Cellular perception of growth rate and the mechanistic origin of bacterial growth law.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Co-crystalization reveals the interaction between AtYchF1 and ppGpp.Frontiers in molecular biosciences · 2022Article
- Article
- Oryza sativa ObgC1 Acts as a Key Regulator of DNA Replication and Ribosome Biogenesis in Chloroplast Nucleoids.Rice (New York, N.Y.) · 2021Article
- The RelA hydrolase domain acts as a molecular switch for (p)ppGpp synthesis.Communications biology · 2021Article
- Regulation ofFrontiers in microbiology · 2021Article
- The Mla pathway ineLife · 2020Article
- GTP Binding is Necessary for the Activation of a Toxic Mutant Isoform of the Essential GTPase ObgE.International journal of molecular sciences · 2019Article
- RSH enzyme diversity for (p)ppGpp metabolism in Phaeodactylum tricornutum and other diatoms.Scientific reports · 2019Article
- Make and break the alarmone: regulation of (p)ppGpp synthetase/hydrolase enzymes in bacteria.FEMS microbiology reviews · 2019Review
- Evolutionary Changes after Translational Challenges Imposed by Horizontal Gene Transfer.Genome biology and evolution · 2019Article
- Human GTPBP10 is required for mitoribosome maturation.Nucleic acids research · 2018Article
- The human Obg protein GTPBP10 is involved in mitoribosomal biogenesis.Nucleic acids research · 2018Article
- Transcriptional Responses to ppGpp and DksA.Annual review of microbiology · 2018Review
- RiceG3 (Bethesda, Md.) · 2018Article
9 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
CgtA(E)/Obg(E)/YhbZ is an Escherichia coli guanine nucleotide binding protein of the Obg/GTP1 subfamily whose members have been implicated in a number of cellular functions including GTP-GDP sensing, sporulation initiation, and translation. Here we describe a kinetic analysis of CgtA(E) with guanine nucleotides and show that its properties are similar to those of the Caulobacter crescentus homolog CgtA(C). CgtA(E) binds both GTP and GDP with moderate affinity, shows high guanine nucleotide exchange rate constants for both nucleotides, and has a relatively low GTP hydrolysis rate. We show that CgtA(E) is associated predominantly with the 50S ribosomal subunit. Interestingly, CgtA(E) copurifies with SpoT, a ribosome-associated ppGpp hydrolase/synthetase involved in the stress response. The interaction between CgtA(E) and SpoT was confirmed by reciprocal coprecipitation experiments and by two-hybrid assays. These studies raise the possibility that the ribosome-associated CgtA(E) is involved in the SpoT-mediated stress response.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.