ArticleTAG. Theoretical and applied genetics. Theoretische und angewandte Genetik2004
Development and assessment of microarray-based DNA fingerprinting in Eucalyptus grandis.
Article in TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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16 citing papers in PubMed, 47 citations in OpenAlex.
- Characterizing Palestinian snake melon (Cucumis melo var. flexuosus) germplasm diversity and structure using SNP and DArTseq markers.BMC plant biology · 2018Article
- Importance of genetic diversity assessment in crop plants and its recent advances: an overview of its analytical perspectives.Genetics research international · 2015Review
- DNA fingerprinting in botany: past, present, future.Investigative genetics · 2014Article
- A gDNA microarray for genotyping salvia species.Molecular biotechnology · 2013Article
- Bin mapping of tomato diversity array (DArT) markers to genomic regions of Solanum lycopersicum × Solanum pennellii introgression lines.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2012Article
- Fingerprinting the Asterid species using subtracted diversity array reveals novel species-specific sequences.PloS one · 2012Article
- Array-based techniques for fingerprinting medicinal herbs.Chinese medicine · 2011Article
- The first genetic map of pigeon pea based on diversity arrays technology (DArT) markers.Journal of genetics · 2011Article
- High-throughput identification of genetic markers using representational oligonucleotide microarray analysis.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2010Article
- Development and assessment of Diversity Arrays Technology for high-throughput DNA analyses in Musa.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2009Article
- A microarray-based method for the parallel analysis of genotypes and expression profiles of wood-forming tissues in Eucalyptus grandis.BMC biotechnology · 2009Article
- Diversity arrays technology (DArT) for pan-genomic evolutionary studies of non-model organisms.PloS one · 2008Article
- A DArT platform for quantitative bulked segregant analysis.BMC genomics · 2007Article
- Diversity arrays technology (DArT) for high-throughput profiling of the hexaploid wheat genome.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2006Article
- A microsatellite-based consensus linkage map for species of Eucalyptus and a novel set of 230 microsatellite markers for the genus.BMC plant biology · 2006Article
- A high-density consensus map of barley linking DArT markers to SSR, RFLP and STS loci and agricultural traits.BMC genomics · 2006Article
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Authors and funding
5 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Development of improved Eucalyptus genotypes involves the routine identification of breeding stock and superior clones. Currently, microsatellites and random amplified polymorphic DNA markers are the most widely used DNA-based techniques for fingerprinting of these trees. While these techniques have provided rapid and powerful fingerprinting assays, they are constrained by their reliance on gel or capillary electrophoresis, and therefore, relatively low throughput of fragment analysis. In contrast, recently developed microarray technology holds the promise of parallel analysis of thousands of markers in plant genomes. The aim of this study was to develop a DNA fingerprinting chip for Eucalyptus grandis and to investigate its usefulness for fingerprinting of eucalypt trees. A prototype chip was prepared using a partial genomic library from total genomic DNA of 23 E. grandis trees, of which 22 were full siblings. A total of 384 cloned genomic fragments were individually amplified and arrayed onto glass slides. DNA fingerprints were obtained for 17 individuals by hybridizing labeled genome representations of the individual trees to the 384-element chip. Polymorphic DNA fragments were identified by evaluating the binary distribution of their background-corrected signal intensities across full-sib individuals. Among 384 DNA fragments on the chip, 104 (27%) were found to be polymorphic. Hybridization of these polymorphic fragments was highly repeatable (R2>0.91) within the E. grandis individuals, and they allowed us to identify all 17 full-sib individuals. Our results suggest that DNA microarrays can be used to effectively fingerprint large numbers of closely related Eucalyptus trees.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.