ArticleThe Journal of cell biology2004
Active Rho is localized to podosomes induced by oncogenic Src and is required for their assembly and function.
Article in The Journal of cell biology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 64 papers.
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Who cites it
64 citing papers in PubMed.
- Article
- Pan-cancer multi-omics dissection of podosome-related genes reveals their dual roles in tumor invasion and immune modulation.Discover oncology · 2026Article
- Chemo-mechanical diffusion waves explain collective dynamics of immune cell podosomes.Nature communications · 2023Article
- Maturation of a postsynaptic domain: Role of small Rho GTPases in organising nicotinic acetylcholine receptor aggregates at the vertebrate neuromuscular junction.Journal of anatomy · 2022Review
- Plectin promotes tumor formation by B16 mouse melanoma cells via regulation of Rous sarcoma oncogene activity.BMC cancer · 2022Article
- Ubiquitous membrane-bound DNase activity in podosomes and invadopodia.The Journal of cell biology · 2021Article
- Nck adapter proteins promote podosome biogenesis facilitating extracellular matrix degradation and cancer invasion.Cancer medicine · 2019Article
- Article
- Differential role for PAK1 and PAK4 during the invadopodia lifecycle.Small GTPases · 2019Article
- A CaThe Journal of cell biology · 2018Article
- Cobalt (II) ions and nanoparticles induce macrophage retention by ROS-mediated down-regulation of RhoA expression.Acta biomaterialia · 2018Article
- Piezo2 channel regulates RhoA and actin cytoskeleton to promote cell mechanobiological responses.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- PAK4 suppresses PDZ-RhoGEF activity to drive invadopodia maturation in melanoma cells.Oncotarget · 2016Article
- The Rho guanine nucleotide exchange factor ARHGEF5 promotes tumor malignancy via epithelial-mesenchymal transition.Oncogenesis · 2016Article
- Lysophosphatidic acid activates the RhoA and NF-κB through Akt/IκBα signaling and promotes prostate cancer invasion and progression by enhancing functional invadopodia formation.Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine · 2016Article
- How to make a static cytokinetic furrow out of traveling excitable waves.Small GTPases · 2016Review
- RTVP-1 regulates glioma cell migration and invasion via interaction with N-WASP and hnRNPK.Oncotarget · 2015Article
- Phosphorylation of Nonmuscle myosin II-A regulatory light chain resists Sendai virus fusion with host cells.Scientific reports · 2015Article
- Voltage-gated sodium channel as a target for metastatic risk reduction with re-purposed drugs.F1000Research · 2015Review
- Decipher the dynamic coordination between enzymatic activity and structural modulation at focal adhesions in living cells.Scientific reports · 2014Article
4 more citing papers are in PubMed but not listed here.
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4 authors.
Funding
Abstract
Transformation of fibroblasts by oncogenic Src causes disruption of actin stress fibers and formation of invasive adhesions called podosomes. Because the small GTPase Rho stimulates stress fiber formation, Rho inactivation by Src has been thought to be necessary for stress fiber disruption. However, we show here that Rho[GTP] levels do not decrease after transformation by activated Src. Inactivation of Rho in Src-transformed fibroblasts by dominant negative RhoA or the Rho-specific inhibitor C3 exoenzyme disrupted podosome structure as judged by localization of podosome components F-actin, cortactin, and Fish. Inhibition of Rho strongly inhibited Src-induced proteolytic degradation of the extracellular matrix. Furthermore, development of an in situ Rho[GTP] affinity assay allowed us to detect endogenous Rho[GTP] at podosomes, where it colocalized with F-actin, cortactin, and Fish. Therefore, Rho is not globally inactivated in Src-transformed fibroblasts, but is necessary for the assembly and function of structures implicated in tumor cell invasion.
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