Evidence map›Paper›PMID 15289478›Full record

ArticleGenome research2004

Fosmid-based physical mapping of the Histoplasma capsulatum genome.

Vincent Magrini, Wesley C Warren, John Wallis, William E Goldman, Jian Xu, Elaine R Mardis, John D McPherson

Open access · bronzeAbstract read
In one paragraph

Article in Genome research, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed, 29 citations in OpenAlex.

  1. Genotypic diversity, virulence, and molecular genetic tools inMicrobiology and molecular biology reviews : MMBR · 2024
    Review
  2. Genetic Diversity of Human Fungal Pathogens.Current clinical microbiology reports · 2023
    Article
  3. Article
  4. Article
  5. Article
  6. Curation of characterized glycoside hydrolases of fungal origin.Database : the journal of biological databases and curation · 2011
    Article
  7. Article
  8. Article
  9. Article
  10. Article
  11. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Vincent MagriniWashington University School of Medicine, Genome Sequencing Center, St. Louis, Missouri 63108, USA.
Wesley C Warren
John Wallis
William E Goldman
Jian Xu
Elaine R Mardis
John D McPherson
Washington University in St. Louis · USBaylor College of Medicine · US

Funding

MOLECULAR MECHANISMS OF HISTOPLASMA PATHOGENESISR01AI025584 · NIAID · WASHINGTON UNIVERSITY · PI GOLDMAN, WILLIAM E · 1988 to 2008
$2.8M
Molecular Mechanisms of Histoplasma PathogenesisR56AI025584 · NIAID · UNIV OF NORTH CAROLINA CHAPEL HILL · PI GOLDMAN, WILLIAM E · 2011 to 2011
$370k
NIAID NIH HHS AI25584NIAID NIH HHS R01 AI025584NIAID NIH HHS R56 AI025584
6 · The paper itself

Abstract

A fosmid library representing 10-fold coverage of the Histoplasma capsulatum G217B genome was used to construct a restriction-based physical map. The data obtained from three restriction endonuclease fingerprints, generated from each clone using BamHI, HindIII, and PstI endonucleases, were combined and used in FPC for automatic and manual contig assembly builds. Concomitantly, a whole-genome shotgun (WGS) sequencing of paired-end reads from plasmids and fosmids were assembled with PCAP, providing a predicted genome size of up to 43.5 Mbp and 17% repetitive DNA. Fosmid paired-end sequences in the WGS assembly provide anchoring information to the physical map and result in joining of existing physical map contigs into 84 clusters containing 9551 fosmid clones. Here, we detail mapping the Histoplasma capsulatum genome comprehensively in fosmids, resulting in an efficient paradigm for de novo sequencing that uses a map-assisted whole genome shotgun approach.

Indexed as

Genome, FungalContig MappingDNA FingerprintingGenetic VectorsGenomic LibraryHistoplasmaRestriction Mapping

Identifiers

PMID15289478
PMCPMC509269
OpenAlexW2137667404

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.