ArticleProceedings of the National Academy of Sciences of the United States of America1992
High-efficiency clonal growth of bloodstream- and insect-form Trypanosoma brucei on agarose plates.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
43 citing papers in PubMed.
- Single-cell motile behaviour ofThe European physical journal. E, Soft matter · 2021Article
- Article
- Culturing and Transfection of Pleomorphic Trypanosoma brucei.Methods in molecular biology (Clifton, N.J.) · 2020Article
- The single mitochondrion of the kinetoplastid parasite Crithidia fasciculata is a dynamic network.PloS one · 2018Article
- Exosome secretion affects social motility in Trypanosoma brucei.PLoS pathogens · 2017Article
- Isolation, cultivation and molecular characterization of a new Trypanosoma equiperdum strain in Mongolia.Parasites & vectors · 2016Article
- Article
- Infection Dynamics and Immune Response in a Newly Described Drosophila-Trypanosomatid Association.mBio · 2015Article
- Comparative efficacy of different in vitro cultivation media for Trypanosoma evansi isolated from different mammalian hosts inhabiting different geographical areas of India.Journal of parasitic diseases : official organ of the Indian Society for Parasitology · 2015Article
- Genetic engineering of Trypanosoma (Dutonella) vivax and in vitro differentiation under axenic conditions.PLoS neglected tropical diseases · 2011Article
- Diverse effects on mitochondrial and nuclear functions elicited by drugs and genetic knockdowns in bloodstream stage Trypanosoma brucei.PLoS neglected tropical diseases · 2010Article
- Social motility in african trypanosomes.PLoS pathogens · 2010Article
- Epigenetic regulation in African trypanosomes: a new kid on the block.Nature reviews. Microbiology · 2009Review
- Approaches for functional analysis of flagellar proteins in African trypanosomes.Methods in cell biology · 2009Article
- KMP-11, a basal body and flagellar protein, is required for cell division in Trypanosoma brucei.Eukaryotic cell · 2008Article
- Identification and characterization of two trypanosome TFIIS proteins exhibiting particular domain architectures and differential nuclear localizations.Molecular microbiology · 2008Article
- Identification of a novel chromosomal passenger complex and its unique localization during cytokinesis in Trypanosoma brucei.PloS one · 2008Article
- Identification of a bacterial-like HslVU protease in the mitochondria of Trypanosoma brucei and its role in mitochondrial DNA replication.PLoS pathogens · 2008Article
- Article
- Sterol 14alpha-demethylase as a potential target for antitrypanosomal therapy: enzyme inhibition and parasite cell growth.Chemistry & biology · 2007Article
Corrections and comments
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2 authors.
Funding
Abstract
This report describes a method for growing both bloodstream- and procyclic-form Trypanosoma brucei as colonies on agarose plates. Procyclic colonies, which took 2 weeks to develop, grew with approximately 17% plating efficiency on SDM-79/0.65% agarose supplemented with 20% (vol/vol) conditioned medium. Bloodstream forms were adapted to in vitro growth in liquid HMI-9 medium and then spread on HMI-9/0.65% agarose plates, where they grew to visible colonies in 3-5 days. Plating efficiencies were from 3 to 80%, depending upon the trypanosome variant and experiment. Colonies were proven to be the result of growth from a single cell and contained approximately 10(6) cells at maturity. Colonies were transferred to filters and probed for multicopy and single-copy genes. Potential uses of this method in conjunction with classical and reverse genetic approaches to studying trypanosomes are discussed.
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