ArticleBiochemistry2004
Human glycolipid transfer protein: probing conformation using fluorescence spectroscopy.
Article in Biochemistry, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 32 citations in OpenAlex.
- Use of Bicelle-Generated Lipid Bilayer Vesicle Nanoparticles for In Vitro Measurement of Lipid Intermembrane Transport.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Role of Gltp in Maturation of Oligodendrocytes Under the Regulation of Nkx2.2.Molecular neurobiology · 2023Article
- Emerging roles for human glycolipid transfer protein superfamily members in the regulation of autophagy, inflammation, and cell death.Progress in lipid research · 2020Review
- Measuring Lipid Transfer Protein Activity Using Bicelle-Dilution Model Membranes.Analytical chemistry · 2020Article
- Functional evaluation of tryptophans in glycolipid binding and membrane interaction by HET-C2, a fungal glycolipid transfer protein.Biochimica et biophysica acta. Biomembranes · 2018Article
- Article
- Phosphatidylserine Stimulates Ceramide 1-Phosphate (C1P) Intermembrane Transfer by C1P Transfer Proteins.The Journal of biological chemistry · 2017Article
- Tumor suppressor berberine binds VASP to inhibit cell migration in basal-like breast cancer.Oncotarget · 2016Article
- Sphingolipid transfer proteins defined by the GLTP-fold.Quarterly reviews of biophysics · 2015Article
- The glycolipid transfer protein (GLTP) domain of phosphoinositol 4-phosphate adaptor protein-2 (FAPP2): structure drives preference for simple neutral glycosphingolipids.Biochimica et biophysica acta · 2013Article
- Conformational folding and stability of the HET-C2 glycolipid transfer protein fold: does a molten globule-like state regulate activity?Biochemistry · 2011Article
- Human GLTP: Three distinct functions for the three tryptophans in a novel peripheral amphitropic fold.Biophysical journal · 2010Article
- Structural determination and tryptophan fluorescence of heterokaryon incompatibility C2 protein (HET-C2), a fungal glycolipid transfer protein (GLTP), provide novel insights into glycolipid specificity and membrane interaction by the GLTP fold.The Journal of biological chemistry · 2010Article
- Glycolipid acquisition by human glycolipid transfer protein dramatically alters intrinsic tryptophan fluorescence: insights into glycolipid binding affinity.The Journal of biological chemistry · 2009Article
- Human glycolipid transfer protein (GLTP) genes: organization, transcriptional status and evolution.BMC genomics · 2008Article
- New BODIPY lipid probes for fluorescence studies of membranes.Journal of lipid research · 2007Article
- Glycolipid transfer proteins.Biochimica et biophysica acta · 2007Review
- The liganding of glycolipid transfer protein is controlled by glycolipid acyl structure.PLoS biology · 2006Article
- Molecular fluorescence, phosphorescence, and chemiluminescence spectrometry.Analytical chemistry · 2006Article
- Glycolipid transfer protein interaction with bilayer vesicles: modulation by changing lipid composition.Biophysical journal · 2005Article
Corrections and comments
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Authors and funding
7 authors at 4 institutions in 3 countries.
Funding
Abstract
Glycolipid transfer protein (GLTP) is a soluble 24 kDa protein that selectively accelerates the intermembrane transfer of glycolipids in vitro. Little is known about the GLTP structure and dynamics. Here, we report the cloning of human GLTP and characterize the environment of the three tryptophans (Trps) of the protein using fluorescence spectroscopy. Excitation at 295 nm yielded an emission maximum (lambda(max)) near 347 nm, indicating a relatively polar average environment for emitting Trps. Quenching with acrylamide at physiological ionic strength or with potassium iodide resulted in linear Stern-Volmer plots, suggesting accessibility of emitting Trps to soluble quenchers. Insights into reversible conformational changes accompanying changes in GLTP activity were provided by addition and rapid dilution of urea while monitoring changes in Trp or 1-anilinonaphthalene-8-sulfonic acid fluorescence. Incubation of GLTP with glycolipid liposomes caused a blue shift in the Trp emission maximum but diminished the fluorescence intensity. The blue-shifted emission maximum, centered near 335 nm, persisted after separation of glycolipid liposomes from GLTP, consistent with formation of a GLTP-glycolipid complex at a glycolipid-liganding site containing Trp. The results provide the first insights into human GLTP structural dynamics by fluorescence spectroscopy, including global conformational changes that accompany GLTP folding into an active conformational state as well as more subtle conformational changes that play a role in GLTP-mediated transfer of glycolipids between membranes, and establish a foundation for future studies of membrane rafts using GLTP.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.