ArticlePlant physiology2004
13C labeling reveals chloroplastic and extrachloroplastic pools of dimethylallyl pyrophosphate and their contribution to isoprene formation.
Article in Plant physiology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 88 citations in OpenAlex.
- Leaf spectroscopy as a tool for predicting the presence of isoprene emissions and terpene storage in central Amazon forest trees.Plant methods · 2025Article
- Leaf isoprene and monoterpene emissions vary with fast-slow carbon economics strategies in central Amazon woody species.Frontiers in plant science · 2025Article
- Deoxyxylulose 5-Phosphate Synthase Does Not Play a Major Role in Regulating the Methylerythritol 4-Phosphate Pathway in Poplar.International journal of molecular sciences · 2024Article
- Interactions between leaf phenological type and functional traits drive variation in isoprene emissions in central Amazon forest trees.Frontiers in plant science · 2024Article
- Emission Trade-Off between Isoprene and Other BVOC Components inInternational journal of molecular sciences · 2023Article
- Age effects of Moso bamboo on leaf isoprene emission characteristics.Frontiers in plant science · 2023Article
- Growth condition controls on G-93 parameters of isoprene emission from tropical trees.Journal of plant research · 2021Article
- Responses of isoprene emission and photochemical efficiency to severe drought combined with prolonged hot weather in hybrid Populus.Journal of experimental botany · 2020Article
- Source of 12C in Calvin-Benson cycle intermediates and isoprene emitted from plant leaves fed with 13CO2.The Biochemical journal · 2020Article
- Distinct metabolic pathways drive monoterpenoid biosynthesis in a natural population of Pelargonium graveolens.Journal of experimental botany · 2020Article
- Effect of Drought on the Methylerythritol 4-Phosphate (MEP) Pathway in the Isoprene Emitting ConiferFrontiers in plant science · 2020Article
- Natural rubber biosynthesis in plants, the rubber transferase complex, and metabolic engineering progress and prospects.Plant biotechnology journal · 2019Review
- Alternative Carbon Sources for Isoprene Emission.Trends in plant science · 2018Review
- Isoprene Responses and Functions in Plants Challenged by Environmental Pressures Associated to Climate Change.Frontiers in plant science · 2017Review
- Article
- Bisphosphonate inhibitors reveal a large elasticity of plastidic isoprenoid synthesis pathway in isoprene-emitting hybrid aspen.Plant physiology · 2015Article
- Acclimation of isoprene emission and photosynthesis to growth temperature in hybrid aspen: resolving structural and physiological controls.Plant, cell & environment · 2015Article
- Dynamic balancing of isoprene carbon sources reflects photosynthetic and photorespiratory responses to temperature stress.Plant physiology · 2014Article
- Metabolic flux analysis of plastidic isoprenoid biosynthesis in poplar leaves emitting and nonemitting isoprene.Plant physiology · 2014Article
- Competition between isoprene emission and pigment synthesis during leaf development in aspen.Plant, cell & environment · 2014Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Isoprene emitted from plants is made in chloroplasts from dimethylallyl pyrophosphate (DMAPP). Leaves of Populus nigra and Phragmites australis exposed to (13)CO(2) for 15 min emitted isoprene that was about 90% (13)C, but DMAPP isolated from those leaves was only 28% and 36% (13)C, respectively. The labeled DMAPP is likely to represent chloroplastic DMAPP contributing to isoprene emission. A substantial (13)C labeling was also found in both emission and DMAPP pool of low-emitting, young leaves of Phragmites. This confirms that low emission of young leaves is not caused by absence of chloroplastic DMAPP but rather by enzyme characteristics. A very low (13)C labeling was found in the DMAPP pool and in the residual isoprene emission of leaves previously fed with fosmidomycin to inhibit isoprene formation. This shows that fosmidomycin is a very effective inhibitor of the chloroplastic biosynthetic pathway of isoprene synthesis, that the residual isoprene is formed from extra-chloroplastic sources, and that chloroplastic and extrachloroplastic pathways are not cross-linked, at least following inhibition of the chloroplastic pathway. Refixation of unlabeled respiratory CO(2) in the light may explain incomplete labeling of isoprene emission, as we found a good association between these two parameters.
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