ArticlePlant physiology2004
Brassinosteroid deficiency due to truncated steroid 5alpha-reductase causes dwarfism in the lk mutant of pea.
Article in Plant physiology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 63 citations in OpenAlex.
- Manipulating brassinosteroid signaling pathway to genetically improve horticultural plants.aBIOTECH · 2025Review
- Identification of Dwarfing Candidate Genes inPlants (Basel, Switzerland) · 2024Article
- Evolution, classification, structure, and functional diversification of steroid 5α-reductase family in eukaryotes.Heliyon · 2024Review
- Biosynthesis and the Roles of Plant Sterols in Development and Stress Responses.International journal of molecular sciences · 2022Review
- Brassinosteroids Benefit Plants Performance by Augmenting Arbuscular Mycorrhizal Symbiosis.Microbiology spectrum · 2021Article
- Melon short internode (CmSi) encodes an ERECTA-like receptor kinase regulating stem elongation through auxin signaling.Horticulture research · 2020Article
- Brassinosteroids play multiple roles in nodulation of pea via interactions with ethylene and auxin.Planta · 2020Article
- A mutation in the intron splice acceptor site of a GA3ox gene confers dwarf architecture in watermelon (Citrullus lanatus L.).Scientific reports · 2020Article
- Regulation of Brassinosteroid Homeostasis in Higher Plants.Frontiers in plant science · 2020Review
- Brassinosteroid overproduction improves lignocellulose quantity and quality to maximize bioethanol yield under green-like biomass process in transgenic poplar.Biotechnology for biofuels · 2020Article
- A Single Nucleotide Deletion in an ABC Transporter Gene Leads to a Dwarf Phenotype in Watermelon.Frontiers in plant science · 2019Article
- Unraveling the Initial Plant Hormone Signaling, Metabolic Mechanisms and Plant Defense Triggering the Endomycorrhizal Symbiosis Behavior.Frontiers in plant science · 2018Review
- Comparative transcriptome analysis provides insights into dwarfism in cherry tomato (Solanum lycopersicum var. cerasiforme).PloS one · 2018Article
- A mutant in the CsDET2 gene leads to a systemic brassinosteriod deficiency and super compact phenotype in cucumber (Cucumis sativus L.).TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2017Article
- Interactions between ethylene, gibberellins, and brassinosteroids in the development of rhizobial and mycorrhizal symbioses of pea.Journal of experimental botany · 2016Article
- The potential roles of strigolactones and brassinosteroids in the autoregulation of nodulation pathway.Annals of botany · 2014Article
- Brassinosteroid biosynthesis and signalling in Petunia hybrida.Journal of experimental botany · 2013Article
- Boosting crop yields with plant steroids.The Plant cell · 2012Review
- TCP1 modulates brassinosteroid biosynthesis by regulating the expression of the key biosynthetic gene DWARF4 in Arabidopsis thaliana.The Plant cell · 2010Article
- The hormonal regulation of de-etiolation.Planta · 2008Article
Corrections and comments
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Authors and funding
14 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The endogenous brassinosteroids in the dwarf mutant lk of pea (Pisum sativum) were quantified by gas chromatography-selected ion monitoring. The levels of castasterone, 6-deoxocastasterone, and 6-deoxotyphasterol in lk shoots were reduced 4-, 70-, and 6-fold, respectively, compared with those of the wild type. The fact that the application of brassinolide restored the growth of the mutant indicated that the dwarf mutant lk is brassinosteroid deficient. Gas chromatography-selected ion monitoring analysis of the endogenous sterols in lk shoots revealed that the levels of campestanol and sitostanol were reduced 160- and 10-fold, respectively, compared with those of wild-type plants. These data, along with metabolic studies, showed that the lk mutant has a defect in the conversion of campest-4-en-3-one to 5alpha-campestan-3-one, which is a key hydrogenation step in the synthesis of campestanol from campesterol. This defect is the same as that found in the Arabidopsis det2 mutant and the Ipomoea nil kbt mutant. The pea gene homologous to the DET2 gene, PsDET2, was cloned, and it was found that the lk mutation would result in a putative truncated PsDET2 protein. Thus it was concluded that the short stature of the lk mutant is due to a defect in the steroidal 5alpha-reductase gene. This defect was also observed in the callus induced from the lk mutant. Biosynthetic pathways involved in the conversion of campesterol to campestanol are discussed in detail.
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