ArticleWorld journal of gastroenterology2004
Prolongation of liver allograft survival by dendritic cells modified with NF-kappaB decoy oligodeoxynucleotides.
Article in World journal of gastroenterology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed, 30 citations in OpenAlex.
- Decoy oligonucleotides targeting NF-κB: a promising therapeutic approach for inflammatory diseases.Inflammation research : official journal of the European Histamine Research Society ... [et al.] · 2025Review
- Amelioration of collagen-induced arthritis using antigen-loaded dendritic cells modified with NF-κB decoy oligodeoxynucleotides.Drug design, development and therapy · 2017Article
- Construction and evaluation of rats' tolerogenic dendritic cells (DC) induced by NF-κB Decoy method.African health sciences · 2014Article
- Polylactide-cyclosporin A nanoparticles for targeted immunosuppression.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2010Article
- Inhibition of NF-kappa B during human dendritic cell differentiation generates anergy and regulatory T-cell activity for one but not two human leukocyte antigen DR mismatches.Human immunology · 2007Article
- Preventing autoimmune arthritis using antigen-specific immature dendritic cells: a novel tolerogenic vaccine.Arthritis research & therapy · 2006Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
aimTo induce the tolerance of rat liver allograft by dendritic cells (DCs) modified with NF-kappaB decoy oligodeoxynucleotides (ODNs).
methodsBone marrow (BM)-derived DCs from SD rats were propagated in the presence of GM-CSF or GM-CSF+IL-4 to obtain immature DCs or mature DCs. GM-CSF+IL-4-propagated DCs were treated with double-strand NF-kappaB decoy ODNs containing two NF-kappaB binding sites or scrambled ODNs to ascertain whether NF-kappaB decoy ODNs might prevent DC maturation. GM-CSF-propagated DCs, GM-CSF+NF-kappaB decoy ODNs or scrambled ODNs-propagated DCs were treated with LPS for 18 h to determine whether NF-kappaB decoy ODNs could prevent LPS-induced IL-12 production in DCs. NF-kappaB binding activities, costimulatory molecule (CD40, CD80, CD86) surface expression, IL-12 protein expression and allostimulatory capacity of DCs were measured with electrophoretic mobility shift assay (EMSA), flow cytometry, Western blotting, and mixed lymphocyte reaction (MLR), respectively. GM-CSF-propagated DCs, GM-CSF+IL-4 -propagated DCs, and GM-CSF+NF-kappaB decoy ODNs or scrambled ODNs-propagated DCs were injected intravenously into recipient LEW rats 7 d prior to liver transplantation and immediately after liver transplantation. Histological grading of liver graft rejection was determined 7 d after liver transplantation. Expression of IL-2, IL-4 and IFN-gamma mRNA in liver graft and in recipient spleen was analyzed by semiquantitative RT-PCR. Apoptosis of liver allograft-infiltrating cells was measured with TUNEL staining.
resultsGM-CSF-propagated DCs, GM-CSF+NF-kappaB decoy ODNs-propagated DCs and GM-CSF+ scrambled ODNs-propagated DCs exhibited features of immature DCs, with similar low level of costimulatory molecule (CD40, CD80, CD86) surface expression, absence of NF-kappaB activation, and few allocostimulatory activities. GM-CSF+IL-4-propagated DCs displayed features of mature DCs, with high levels of costimulatory molecule (CD40, CD80, CD86) surface expression, marked NF-kappaB activation, and significant allocostimulatory activity. NF-kappaB decoy ODNs completely abrogated IL-4-induced DC maturation and allocostimulatory activity as well as LPS-induced NF-kappaB activation and IL-12 protein expression in DCs. GM-CSF+NF-kappaB decoy ODNs-propagated DCs promoted apoptosis of liver allograft-infiltrating cells within portal areas, and significantly decreased the expression of IL-2 and IFN-gamma mRNA but markedly elevated IL-4 mRNA expression both in liver allograft and in recipient spleen, and consequently suppressed liver allograft rejection, and promoted liver allograft survival.
conclusionNF-kappaB decoy ODNs-modified DCs can prolong liver allograft survival by promoting apoptosis of graft-infiltrating cells within portal areas as well as down-regulating IL-2 and IFN-gamma mRNA and up-regulating IL-4 mRNA expression both in liver graft and in recipient spleen.
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