ArticleThe Journal of biological chemistry2004
Molecular and functional analyses of two new calcium-activated chloride channel family members from mouse eye and intestine.
Article in The Journal of biological chemistry, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
21 citing papers in PubMed, 48 citations in OpenAlex.
- Article
- Article
- Comparative Transcriptome Analysis During the Seven Developmental Stages of Channel Catfish (Frontiers in genetics · 2020Article
- Chloride - The Underrated Ion in Nociceptors.Frontiers in neuroscience · 2020Review
- Stat3 modulates chloride channel accessory protein expression in normal and neoplastic mammary tissue.Cell death & disease · 2016Article
- Naturally Occurring Deletion Mutants of the Pig-Specific, Intestinal Crypt Epithelial Cell Protein CLCA4b without Apparent Phenotype.PloS one · 2015Article
- Self-cleavage of human CLCA1 protein by a novel internal metalloprotease domain controls calcium-activated chloride channel activation.The Journal of biological chemistry · 2012Article
- The porcine chloride channel calcium-activated family member pCLCA4a mirrors lung expression of the human hCLCA4.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2012Article
- Article
- Isolation and Expression Profile of the Ca-Activated Chloride Channel-like Membrane Protein 6 Gene in Xenopus laevis.Laboratory animal research · 2011Article
- Cellular distribution and subcellular localization of mCLCA1/2 in murine gastrointestinal epithelia.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2010Article
- Murine mCLCA5 is expressed in granular layer keratinocytes of stratified epithelia.Histochemistry and cell biology · 2010Article
- Loss of Ca-mediated ion transport during colitis correlates with reduced ion transport responses to a Ca-activated K channel opener.British journal of pharmacology · 2009Article
- Expression analysis of the CLCA gene family in mouse and human with emphasis on the nervous system.BMC developmental biology · 2009Article
- The role of CLCA proteins in inflammatory airway disease.Annual review of physiology · 2009Review
- Murine mCLCA6 is an integral apical membrane protein of non-goblet cell enterocytes and co-localizes with the cystic fibrosis transmembrane conductance regulator.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2008Article
- Cholinergic regulation of epithelial ion transport in the mammalian intestine.British journal of pharmacology · 2006Review
- Differential expression of calcium-activated chloride channels (CLCA) gene family members in the small intestine of cystic fibrosis mouse models.Histochemistry and cell biology · 2006Article
- Genetic segregation of airway disease traits despite redundancy of calcium-activated chloride channel family members.Physiological genomics · 2006Article
- Diversity of Cl(-) channels.Cellular and molecular life sciences : CMLS · 2006Review
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
Two new calcium-activated chloride channel (CLCA) family members, mCLCA5 and mCLCA6, have been cloned from mouse eye and intestine, respectively. mCLCA5 is highly homologous to hCLCA2, and mCLCA6 is highly homologous to hCLCA4. mCLCA5 is widely expressed with strong expression in eye and spleen, whereas mCLCA6 is primarily expressed in intestine and stomach. mCLCA6 is also expressed as a splice variant lacking exon 8 and part of exon 10 in intestine and stomach. Transfection of tsA201 cells with enhanced green fluorescent protein-tagged versions of the three cDNAs reveals protein products of 155 and 65 kDa for mCLCA5 and mCLCA6 and 145 and 65 kDa for the mCLCA6 splice variant. In vitro translation of mCLCA5 generates a 90-kDa protein that does not appear to be glycosylated. mCLCA6 also generates a 90-kDa protein that is glycosylated to a 110-kDa product, whereas the mCLCA6 splice variant generates an 80-kDa product that is 100 kDa after glycosylation. Treatment of enhanced green fluorescent protein-tagged mCLCA6 with PNGase F (peptide: N-glycosidase F) to remove N-linked glycosyl groups shows a reduction in size of the 65 kDa product to 60 kDa. Consistent with the hypothesis that mCLCA5, mCLCA6, and its splice variant encode calcium-activated chloride channels, in HEK293 cells expressing CLCAs ionomycin-evoked increases in intracellular calcium stimulated a current that reversed near Cl(-) equilibrium potential, E(Cl). Furthermore, these currents were inhibited by the chloride channel blocker niflumic acid. Given the prominent role of hCLCA2 in cancer cell adhesion and the unique high level of expression of hCLCA4 in brain, the identification of their murine counterparts presents the opportunity to clarify the role of CLCAs in disease and normal cell physiology.
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