ArticleThe Biochemical journal2004
Ubiquitination of the peroxisomal import receptor Pex5p.
Article in The Biochemical journal, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 65 papers.
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Who cites it
65 citing papers in PubMed, 165 citations in OpenAlex.
- Pex6 and ubiquitination regulate topological remodeling of the peroxisomal membrane protein Pex14.The Journal of biological chemistry · 2026Article
- Noncanonical and reversible cysteine ubiquitination prevents the overubiquitination of PEX5 at the peroxisomal membrane.PLoS biology · 2024Article
- PEX1 is essential for glycosome biogenesis and trypanosomatid parasite survival.Frontiers in cellular and infection microbiology · 2024Article
- Article
- An engineered variant of MECR reductase reveals indispensability of long-chain acyl-ACPs for mitochondrial respiration.Nature communications · 2023Article
- Molecular basis of the glycosomal targeting of PEX11 and its mislocalization to mitochondrion in trypanosomes.Frontiers in cell and developmental biology · 2023Article
- Insights into the Structure and Function of the Pex1/Pex6 AAA-ATPase in Peroxisome Homeostasis.Cells · 2022Review
- Ubiquitin-conjugating activity by PEX4 is required for efficient protein transport to peroxisomes in Arabidopsis thaliana.The Journal of biological chemistry · 2022Article
- Non-lysine ubiquitylation: Doing things differently.Frontiers in molecular biosciences · 2022Review
- Post-translational modifications of proteins associated with yeast peroxisome membrane: An essential mode of regulatory mechanism.Genes to cells : devoted to molecular & cellular mechanisms · 2021Review
- A hunt for OM45 synthetic petite interactions in Saccharomyces cerevisiae reveals a role for Miro GTPase Gem1p in cristae structure maintenance.MicrobiologyOpen · 2021Article
- Genetic dissection of the mitochondrial lipoylation pathway in yeast.BMC biology · 2021Article
- Autophagy Stimulus-Dependent Role of the Small GTPase Ras2 in Peroxisome Degradation.Biomolecules · 2020Article
- The Peroxisomal PTS1-Import Defect ofInternational journal of molecular sciences · 2020Article
- Pexophagy: A Model for Selective Autophagy.International journal of molecular sciences · 2020Review
- Pex14p Phosphorylation Modulates Import of Citrate Synthase 2 Into Peroxisomes inFrontiers in cell and developmental biology · 2020Article
- Ceramide regulates interaction of Hsd17b4 with Pex5 and function of peroxisomes.Biochimica et biophysica acta. Molecular and cell biology of lipids · 2019Article
- Vps10-mediated targeting of Pep4 determines the activity of the vacuole in a substrate-dependent manner.Scientific reports · 2019Article
- Article
- Current Advances in Protein Import into Peroxisomes.The protein journal · 2019Review
5 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Proteins harbouring a peroxisomal targeting signal of type 1 (PTS1) are recognized by the import receptor Pex5p in the cytosol which directs them to a docking and translocation complex at the peroxisomal membrane. We demonstrate the ubiquitination of Pex5p in cells lacking components of the peroxisomal AAA (ATPases associated with various cellular activities) or Pex4p-Pex22p complexes of the peroxisomal protein import machinery and in cells affected in proteasomal degradation. In cells lacking components of the Pex4p-Pex22p complex, mono-ubiquitinated Pex5p represents the major modification, while in cells lacking components of the AAA complex polyubiquitinated forms are most prominent. Ubiquitination of Pex5p is shown to take place exclusively at the peroxisomal membrane after the docking step, and requires the presence of the RING-finger peroxin Pex10p. Mono- and poly-ubiquitination are demonstrated to depend on the ubiquitin-conjugating enzyme Ubc4p, suggesting that the ubiquitinated forms of Pex5p are targeted for proteasomal degradation. Accumulation of ubiquitinated Pex5p in proteasomal mutants demonstrates that the ubiquitination of Pex5p also takes place in strains which are not affected in peroxisomal biogenesis, indicating that the ubiquitination of Pex5p represents a genuine stage in the Pex5p receptor cycle.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.