ArticleThe EMBO journal2004
True reversal of Mu integration.
Article in The EMBO journal, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
13 citing papers in PubMed.
- Ultralow-input single-tube linked-read library method enables short-read second-generation sequencing systems to routinely generate highly accurate and economical long-range sequencing information.Genome research · 2020Article
- Structure of a P element transposase-DNA complex reveals unusual DNA structures and GTP-DNA contacts.Nature structural & molecular biology · 2019Article
- Mu-driven transposition of recombinant mini-Mu unit DNA in the Corynebacterium glutamicum chromosome.Applied microbiology and biotechnology · 2018Article
- Target DNA bending by the Mu transpososome promotes careful transposition and prevents its reversal.eLife · 2017Article
- P Transposable Elements in Drosophila and other Eukaryotic Organisms.Microbiology spectrum · 2015Review
- Article
- Transposable Phage Mu.Microbiology spectrum · 2014Review
- Article
- Architecture of the Tn7 posttransposition complex: an elaborate nucleoprotein structure.Journal of molecular biology · 2010Article
- The dynamic Mu transpososome: MuB activation prevents disintegration.Journal of molecular biology · 2007Article
- Control of transposase activity within a transpososome by the configuration of the flanking DNA segment of the transposon.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- Characteristics of MuA transposase-catalyzed processing of model transposon end DNA hairpin substrates.Nucleic acids research · 2006Article
- Role of metal ions in catalysis by HIV integrase analyzed using a quantitative PCR disintegration assay.Nucleic acids research · 2006Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
Abstract
We describe a high-temperature (75 degrees C) transition in the Mu integration complex that causes efficient and true reversal of the integration reaction. A second reversal pathway, first described as 'foldback' reversal for the HIV integrase, was also observed upon disassembly/reassembly of the Mu complex at normal temperatures. Both true and foldback reversal severed only one or the other of the two integrated Mu ends, and each exhibited distinct metal ion specificities. Our results directly implicate an altered transposase configuration in the Mu strand transfer complex that inhibits reversal, thereby regulating the directionality of transposition.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.