ArticleProceedings of the National Academy of Sciences of the United States of America2004
An antiproliferative factor from interstitial cystitis patients is a frizzled 8 protein-related sialoglycopeptide.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 72 papers.
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Who cites it
72 citing papers in PubMed, 180 citations in OpenAlex.
- Impact of intravesical hyaluronic acid and chondroitin sulfate on bladder pain syndrome/interstitial cystitis.International urogynecology journal · 2012Trial
- Signaling pathways related to interstitial cystitis.Frontiers in immunology · 2026Review
- Mendelian Disorders in an Interstitial Cystitis/Bladder Pain Syndrome Cohort.Advanced genetics (Hoboken, N.J.) · 2023Article
- The effect of a methyl group on structure and function: Serine vs. threonine glycosylation and phosphorylation.Frontiers in molecular biosciences · 2023Review
- Peptidomics analysis reveals changes in small urinary peptides in patients with interstitial cystitis/bladder pain syndrome.Scientific reports · 2022Article
- Transient receptor potential vanilloid type 4 (TRPV4) in urinary bladder structure and function.Current topics in membranes · 2022Review
- National prevalence of IC/BPS in women and men utilizing veterans health administration data.Frontiers in pain research (Lausanne, Switzerland) · 2022Article
- Prevalence, Risk Factors, Pathophysiology, Potential Biomarkers and Management of Feline Idiopathic Cystitis: An Update Review.Frontiers in veterinary science · 2022Review
- Urinary Biomarkers in Interstitial Cystitis/Bladder Pain Syndrome and Its Impact on Therapeutic Outcome.Diagnostics (Basel, Switzerland) · 2021Review
- Components of the Endogenous Cannabinoid System as Potential Biomarkers for Interstitial Cystitis/Bladder Pain Syndrome.Diagnostics (Basel, Switzerland) · 2021Review
- Purinergic signalling in the urinary bladder - When function becomes dysfunction.Autonomic neuroscience : basic & clinical · 2021Article
- Acyloxyacyl hydrolase is a host determinant of gut microbiome-mediated pelvic pain.American journal of physiology. Regulatory, integrative and comparative physiology · 2021Article
- Analysis of key genes and micro-RNA-mRNA regulatory networks in women with ulcerative interstitial cystitis/pain bladder syndrome.International urogynecology journal · 2019Article
- Article
- Antiproliferative factor (APF) binds specifically to sites within the cytoskeleton-associated protein 4 (CKAP4) extracellular domain.BMC biochemistry · 2017Article
- Sleeping beauty: awakening urothelium from its slumber.American journal of physiology. Renal physiology · 2017Review
- Urinary proteomics and metabolomics studies to monitor bladder health and urological diseases.BMC urology · 2016Review
- Implications for bidirectional signaling between afferent nerves and urothelial cells-ICI-RS 2014.Neurourology and urodynamics · 2016Review
- Influence of polyunsaturated fatty acids on urologic inflammation.International urology and nephrology · 2015Review
- How many lives does CLIMP-63 have?Biochemical Society transactions · 2015Review
12 more citing papers are in PubMed but not listed here.
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
Abstract
Approximately 1 million people in the United States suffer from interstitial cystitis, a chronic painful urinary bladder disorder characterized by thinning or ulceration of the bladder epithelial lining; its etiology is unknown. We have identified a glycosylated frizzled-related peptide inhibitor of cell proliferation that is secreted specifically by bladder epithelial cells from patients with this disorder. This antiproliferative factor (APF) profoundly inhibits bladder cell proliferation by means of regulation of cell adhesion protein and growth factor production. The structure of APF was deduced by using ion trap mass spectrometry (MS), enzymatic digestion, lectin affinity chromatography, and total synthesis, and confirmed by coelution of native and synthetic APF derivatives on microcapillary reversed-phase liquid chromatography (microRPLC)/MS. APF was determined to be an acidic, heat-stable sialoglycopeptide whose peptide chain has 100% homology to the putative sixth transmembrane domain of frizzled 8. Both synthetic and native APF had identical biological activity in normal bladder epithelial cells and T24 bladder cancer cells. Northern blot analysis indicated binding of a probe containing the sequence for the frizzled 8 segment with mRNA extracted from cells of patients with interstitial cystitis but not controls. APF is therefore a frizzled-related peptide growth inhibitor shown to contain exclusively a transmembrane segment of a frizzled protein and is a potential biomarker for interstitial cystitis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.