ArticleMolecular biology of the cell2004
Visualization of regulated exocytosis with a granule-membrane probe using total internal reflection microscopy.
Article in Molecular biology of the cell, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 55 papers.
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Who cites it
55 citing papers in PubMed, 130 citations in OpenAlex.
- Exosome secretion kinetics are controlled by temperature.Biophysical journal · 2023Article
- Imaging nanoscale axial dynamics at the basal plasma membrane.The international journal of biochemistry & cell biology · 2023Review
- Caveolin-initiated macropinocytosis is required for efficient silica nanoparticles' transcytosis across the alveolar epithelial barrier.Scientific reports · 2022Article
- VAMP2 and synaptotagmin mobility in chromaffin granule membranes: implications for regulated exocytosis.Molecular biology of the cell · 2022Article
- Imaging vesicle formation dynamics supports the flexible model of clathrin-mediated endocytosis.Nature communications · 2022Article
- Real-Time Measurement of the Hygroscopic Growth Dynamics of Single Aerosol Nanoparticles with Bloch Surface Wave Microscopy.ACS nano · 2020Article
- Integrating Optical and Electrochemical Approaches to Assess the Actions of Dynamin at the Fusion Pore.Methods in molecular biology (Clifton, N.J.) · 2020Article
- Label-free surface-sensitive photonic microscopy with high spatial resolution using azimuthal rotation illumination.Science advances · 2019Article
- The fusion pore, 60 years after the first cartoon.FEBS letters · 2018Review
- The high-affinity calcium sensor synaptotagmin-7 serves multiple roles in regulated exocytosis.The Journal of general physiology · 2018Review
- Synaptotagmin isoforms confer distinct activation kinetics and dynamics to chromaffin cell granules.The Journal of general physiology · 2017Article
- Evidence for intraventricular secretion of angiotensinogen and angiotensin by the subfornical organ using transgenic mice.American journal of physiology. Regulatory, integrative and comparative physiology · 2017Article
- Munc13-4 functions as a CaMolecular biology of the cell · 2017Article
- Imaging the recruitment and loss of proteins and lipids at single sites of calcium-triggered exocytosis.Molecular biology of the cell · 2016Article
- Article
- Measuring Exocytosis Rate Using Corrected Fluorescence Recovery After Photoconversion.Traffic (Copenhagen, Denmark) · 2016Article
- Granuphilin exclusively mediates functional granule docking to the plasma membrane.Scientific reports · 2016Article
- Real-time fluorescence imaging with 20 nm axial resolution.Nature communications · 2015Article
- Spatiotemporal detection and analysis of exocytosis reveal fusion "hotspots" organized by the cytoskeleton in endocrine cells.Biophysical journal · 2015Article
- Vesicle Motion during Sustained Exocytosis in Chromaffin Cells: Numerical Model Based on Amperometric Measurements.PloS one · 2015Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
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Abstract
Secretory granules labeled with Vamp-green fluorescent protein (GFP) showed distinct signatures upon exocytosis when viewed by total internal reflection fluorescence microscopy. In approximately 90% of fusion events, we observed a large increase in fluorescence intensity coupled with a transition from a small punctate appearance to a larger, spreading cloud with free diffusion of the Vamp-GFP into the plasma membrane. Quantitation suggests that these events reflect the progression of an initially fused and spherical granule flattening into the plane of the plasma membrane as the Vamp-GFP simultaneously diffuses through the fusion junction. Approximately 10% of the events showed a transition from puncta to ring-like structures coupled with little or no spreading. The ring-like images correspond quantitatively to granules fusing and retaining concavity (recess of approximately 200 nm). A majority of fusion events involved granules that were present in the evanescent field for at least 12 s. However, approximately 20% of the events involved granules that were present in the evanescent field for no more than 0.3 s, indicating that the interaction of the granule with the plasma membrane that leads to exocytosis can occur within that time. In addition, approximately 10% of the exocytotic sites were much more likely to occur within a granule diameter of a previous event than can be accounted for by chance, suggestive of sequential (piggy-back) exocytosis that has been observed in other cells. Overall granule behavior before and during fusion is strikingly similar to exocytosis previously described in the constitutive secretory pathway.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.