ArticleMolecular biology of the cell2004
Golgi enzymes are enriched in perforated zones of golgi cisternae but are depleted in COPI vesicles.
Article in Molecular biology of the cell, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed, 93 citations in OpenAlex.
- Models of Intracellular Transport: Contradictions and Current Understanding.Sub-cellular biochemistry · 2026Review
- The 50-nm Free Vesicles Visible inCurrent issues in molecular biology · 2025Article
- MARS2 drives metabolic switch of non-small-cell lung cancer cells via interaction with MCU.Redox biology · 2023Article
- Acute COG complex inactivation unveiled its immediate impact on Golgi and illuminated the nature of intra-Golgi recycling vesicles.Traffic (Copenhagen, Denmark) · 2023Article
- The Diffusion Model of Intra-Golgi Transport Has Limited Power.International journal of molecular sciences · 2023Article
- Comparison of the Cisterna Maturation-Progression Model with the Kiss-and-Run Model of Intra-Golgi Transport: Role of Cisternal Pores and Cargo Domains.International journal of molecular sciences · 2022Article
- Membrane Curvature, Trans-Membrane Area Asymmetry, Budding, Fission and Organelle Geometry.International journal of molecular sciences · 2020Review
- The function of GORASPs in Golgi apparatus organization in vivo.The Journal of cell biology · 2020Article
- Labeling Strategies Matter for Super-Resolution Microscopy: A Comparison between HaloTags and SNAP-tags.Cell chemical biology · 2019Article
- Models of Intracellular Transport: Pros and Cons.Frontiers in cell and developmental biology · 2019Article
- Article
- The tobacco-specific carcinogen-operated calcium channel promotes lung tumorigenesis via IGF2 exocytosis in lung epithelial cells.Nature communications · 2016Article
- Article
- Trans-membrane area asymmetry controls the shape of cellular organelles.International journal of molecular sciences · 2015Article
- Article
- Golgi's way: a long path toward the new paradigm of the intra-Golgi transport.Histochemistry and cell biology · 2013Review
- A three-stage model of Golgi structure and function.Histochemistry and cell biology · 2013Review
- The dynamics of engineered resident proteins in the mammalian Golgi complex relies on cisternal maturation.The Journal of cell biology · 2013Article
- The kiss-and-run model of intra-Golgi transport.International journal of molecular sciences · 2012Article
- Golgi and related vesicle proteomics: simplify to identify.Cold Spring Harbor perspectives in biology · 2011Review
Corrections and comments
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Authors and funding
16 authors at 3 institutions in 3 countries.
Funding
Abstract
In the most widely accepted version of the cisternal maturation/progression model of intra-Golgi transport, the polarity of the Golgi complex is maintained by retrograde transport of Golgi enzymes in COPI-coated vesicles. By analyzing enzyme localization in relation to the three-dimensional ultrastructure of the Golgi complex, we now observe that Golgi enzymes are depleted in COPI-coated buds and 50- to 60-nm COPI-dependent vesicles in a variety of different cell types. Instead, we find that Golgi enzymes are concentrated in the perforated zones of cisternal rims both in vivo and in a cell-free system. This lateral segregation of Golgi enzymes is detectable in some stacks during steady-state transport, but it was significantly prominent after blocking endoplasmic reticulum-to-Golgi transport. Delivery of transport carriers to the Golgi after the release of a transport block leads to a diminution in Golgi enzyme concentrations in perforated zones of cisternae. The exclusion of Golgi enzymes from COPI vesicles and their transport-dependent accumulation in perforated zones argues against the current vesicle-mediated version of the cisternal maturation/progression model.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.