ArticleMolecular and cellular biology2004
RPAP1, a novel human RNA polymerase II-associated protein affinity purified with recombinant wild-type and mutated polymerase subunits.
Article in Molecular and cellular biology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed, 68 citations in OpenAlex.
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- Biogenesis of RNA Polymerases in Yeast.Frontiers in molecular biosciences · 2021Review
- Modeling DNA Opening in the Eukaryotic Transcription Initiation Complexes via Coarse-Grained Models.Frontiers in molecular biosciences · 2021Article
- Methods review: Mass spectrometry analysis of RNAPII complexes.Methods (San Diego, Calif.) · 2019Review
- Gpn2 and Rba50 Directly Participate in the Assembly of the Rpb3 Subcomplex in the Biogenesis of RNA Polymerase II.Molecular and cellular biology · 2018Article
- A Role for MINIYO and QUATRE-QUART2 in the Assembly of RNA Polymerases II, IV, and V in Arabidopsis.The Plant cell · 2018Article
- The RNA Polymerase II Factor RPAP1 Is Critical for Mediator-Driven Transcription and Cell Identity.Cell reports · 2018Article
- R2TP/Prefoldin-like component RUVBL1/RUVBL2 directly interacts with ZNHIT2 to regulate assembly of U5 small nuclear ribonucleoprotein.Nature communications · 2017Article
- RIMA-Dependent Nuclear Accumulation of IYO Triggers Auxin-Irreversible Cell Differentiation in Arabidopsis.The Plant cell · 2017Article
- Chromatin changes in SMARCAL1 deficiency: A hypothesis for the gene expression alterations of Schimke immuno-osseous dysplasia.Nucleus (Austin, Tex.) · 2016Article
- Role of Ostm1 Cytosolic Complex with Kinesin 5B in Intracellular Dispersion and Trafficking.Molecular and cellular biology · 2016Article
- Substrate recognition and function of the R2TP complex in response to cellular stress.Frontiers in genetics · 2015Review
- Nuclear import of RNA polymerase II is coupled with nucleocytoplasmic shuttling of the RNA polymerase II-associated protein 2.Nucleic acids research · 2013Article
- Eukaryotic GPN-loop GTPases paralogs use a dimeric assembly reminiscent of archeal GPN.Cell cycle (Georgetown, Tex.) · 2013Article
- Discovery of cell compartment specific protein-protein interactions using affinity purification combined with tandem mass spectrometry.Journal of proteome research · 2013Article
- A newly uncovered group of distantly related lysine methyltransferases preferentially interact with molecular chaperones to regulate their activity.PLoS genetics · 2013Article
- A two-stage association study identifies methyl-CpG-binding domain protein 2 gene polymorphisms as candidates for breast cancer susceptibility.European journal of human genetics : EJHG · 2012Article
- Penetrance of biallelic SMARCAL1 mutations is associated with environmental and genetic disturbances of gene expression.Human molecular genetics · 2012Article
- Transcriptional regulation by Pol II(G) involving mediator and competitive interactions of Gdown1 and TFIIF with Pol II.Molecular cell · 2012Article
- Human GTPases associate with RNA polymerase II to mediate its nuclear import.Molecular and cellular biology · 2011Article
Corrections and comments
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Authors and funding
18 authors at 4 institutions in 2 countries.
Funding
Abstract
We have programmed human cells to express physiological levels of recombinant RNA polymerase II (RNAPII) subunits carrying tandem affinity purification (TAP) tags. Double-affinity chromatography allowed for the simple and efficient isolation of a complex containing all 12 RNAPII subunits, the general transcription factors TFIIB and TFIIF, the RNAPII phosphatase Fcp1, and a novel 153-kDa polypeptide of unknown function that we named RNAPII-associated protein 1 (RPAP1). The TAP-tagged RNAPII complex is functionally active both in vitro and in vivo. A role for RPAP1 in RNAPII transcription was established by shutting off the synthesis of Ydr527wp, a Saccharomyces cerevisiae protein homologous to RPAP1, and demonstrating that changes in global gene expression were similar to those caused by the loss of the yeast RNAPII subunit Rpb11. We also used TAP-tagged Rpb2 with mutations in fork loop 1 and switch 3, two structural elements located strategically within the active center, to start addressing the roles of these elements in the interaction of the enzyme with the template DNA during the transcription reaction.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.