ArticleMolecular and cellular biology2004
Role of DNA replication proteins in double-strand break-induced recombination in Saccharomyces cerevisiae.
Article in Molecular and cellular biology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 78 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
78 citing papers in PubMed, 131 citations in OpenAlex.
- Repair of single-strand breaks during the cell cycle - saturation of repair capacity.Nucleus (Austin, Tex.) · 2026Article
- ISWI/CHRAC orchestrates sequential chromatin-remodeling activities by mobilizing DNA polymerase ε for homologous recombination.Communications biology · 2026Article
- Mutations and structural variants arising during double-strand break repair.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Recombination-coupled DNA synthesis facilitates post-invasion steps in meiotic crossover and noncrossover formations.Nucleic acids research · 2025Article
- Physical interactions between specifically regulated subpopulations of the MCM and RNR complexes prevent genetic instability.PLoS genetics · 2024Article
- Double-strand break repair-associated intragenic deletions and tandem duplications suggest the architecture of the repair replication fork.bioRxiv : the preprint server for biology · 2023Article
- The structure-specific endonuclease complex SLX4-XPF regulates Tus-Ter-induced homologous recombination.Nature structural & molecular biology · 2022Article
- Article
- Role of RadA and DNA Polymerases in Recombination-Associated DNA Synthesis in Hyperthermophilic Archaea.Biomolecules · 2020Article
- DNA Polymerase Delta Synthesizes Both Strands during Break-Induced Replication.Molecular cell · 2019Article
- Mating-type switching by homology-directed recombinational repair: a matter of choice.Current genetics · 2019Review
- Review
- Homologous recombination and the repair of DNA double-strand breaks.The Journal of biological chemistry · 2018Review
- Repair of a Site-Specific DNA Cleavage: Old-School Lessons for Cas9-Mediated Gene Editing.ACS chemical biology · 2018Review
- Revertant mosaicism in genodermatoses.Cellular and molecular life sciences : CMLS · 2017Review
- Global analysis of genomic instability caused by DNA replication stress in Saccharomyces cerevisiae.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Eukaryotic DNA Polymerases in Homologous Recombination.Annual review of genetics · 2016Review
- Strand displacement synthesis by yeast DNA polymerase ε.Nucleic acids research · 2016Article
- Remarkably Long-Tract Gene Conversion Induced by Fragile Site Instability in Saccharomyces cerevisiae.Genetics · 2016Article
- Article
18 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
Abstract
Mitotic double-strand break (DSB)-induced gene conversion involves new DNA synthesis. We have analyzed the requirement of several essential replication components, the Mcm proteins, Cdc45p, and DNA ligase I, in the DNA synthesis of Saccharomyces cerevisiae MAT switching. In an mcm7-td (temperature-inducible degron) mutant, MAT switching occurred normally when Mcm7p was degraded below the level of detection, suggesting the lack of the Mcm2-7 proteins during gene conversion. A cdc45-td mutant was also able to complete recombination. Surprisingly, even after eliminating both of the identified DNA ligases in yeast, a cdc9-1 dnl4 Delta strain was able to complete DSB repair. Previous studies of asynchronous cultures carrying temperature-sensitive alleles of PCNA, DNA polymerase alpha (Pol alpha), or primase showed that these mutations inhibited MAT switching (A. M. Holmes and J. E. Haber, Cell 96:415-424, 1999). We have reevaluated the roles of these proteins in G(2)-arrested cells. Whereas PCNA was still essential for MAT switching, neither Pol alpha nor primase was required. These results suggest that arresting cells in S phase using ts alleles of Pol alpha-primase, prior to inducing the DSB, sequesters some other component that is required for repair. We conclude that DNA synthesis during gene conversion is different from S-phase replication, involving only leading-strand polymerization.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.