ArticleNucleic acids research2004
Evolution of divergent DNA recognition specificities in VDE homing endonucleases from two yeast species.
Article in Nucleic acids research, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 43 citations in OpenAlex.
- Group I introns: Structure, splicing and their applications in medical mycology.Genetics and molecular biology · 2024Article
- Nuclease genes occupy boundaries of genetic exchange between bacteriophages.NAR genomics and bioinformatics · 2023Article
- Nuclease genes occupy boundaries of genetic exchange between bacteriophages.bioRxiv : the preprint server for biology · 2023Article
- Adaptive Gene Content and Allele Distribution Variations in the Wild and Domesticated Populations ofFrontiers in microbiology · 2021Article
- Structural Basis for the Persistence of Homing Endonucleases in Transcription Factor IIB Inteins.Journal of molecular biology · 2017Article
- Intein Clustering Suggests Functional Importance in Different Domains of Life.Molecular biology and evolution · 2016Article
- Evolution and Application of Inteins inFrontiers in microbiology · 2016Review
- Analysis of inteins in the Candida parapsilosis complex for simple and accurate species identification.Journal of clinical microbiology · 2013Article
- LAHEDES: the LAGLIDADG homing endonuclease database and engineering server.Nucleic acids research · 2012Article
- Article
- Home and away- the evolutionary dynamics of homing endonucleases.BMC evolutionary biology · 2011Article
- Evolutionary maintenance of selfish homing endonuclease genes in the absence of horizontal transfer.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- Efficient targeting of a SCID gene by an engineered single-chain homing endonuclease.Nucleic acids research · 2009Article
- Optimization of in vivo activity of a bifunctional homing endonuclease and maturase reverses evolutionary degradation.Nucleic acids research · 2009Article
- Coevolution of a homing endonuclease and its host target sequence.Journal of molecular biology · 2007Article
- Inteins, introns, and homing endonucleases: recent revelations about the life cycle of parasitic genetic elements.BMC evolutionary biology · 2006Article
- Investigation of the mechanism of meiotic DNA cleavage by VMA1-derived endonuclease uncovers a meiotic alteration in chromatin structure around the target site.Eukaryotic cell · 2006Article
- The distribution and evolutionary history of the PRP8 intein.BMC evolutionary biology · 2006Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Homing endonuclease genes (HEGs) are mobile DNA elements that are thought to confer no benefit to their host. They encode site-specific DNA endonucleases that perpetuate the element within a species population by homing and disseminate it between species by horizontal transfer. Several yeast species contain the VMA1 HEG that encodes the intein-associated VMA1-derived endonuclease (VDE). The evolutionary state of VDEs from 12 species was assessed by assaying their endonuclease activities. Only two enzymes are active, PI-ZbaI from Zygosaccharomyces bailii and PI-ScaI from Saccharomyces cariocanus. PI-ZbaI cleaves the Z.bailii recognition sequence significantly faster than the Saccharomyces cerevisiae site, which differs at six nucleotide positions. A mutational analysis indicates that PI-ZbaI cleaves the S.cerevisiae substrate poorly due to the absence of a contact that is analogous to one made in PI-SceI between Gln-55 and nucleotides +9/+10. PI-ZbaI cleaves the Z.bailii substrate primarily due to a single base-pair substitution (A/T+5 --> T/A+5). Structural modeling of the PI-ZbaI/DNA complex suggests that Arg-331, which is absent in PI-SceI, contacts T/A+5, and the reduced activity observed in a PI-ZbaI R331A mutant provides evidence for this interaction. These data illustrate that homing endonucleases evolve altered specificity as they adapt to recognize alternative target sites.
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