ArticleJournal of virology2004
Caspases mediate processing of the capsid precursor and cell release of human astroviruses.
Article in Journal of virology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 52 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
52 citing papers in PubMed, 103 citations in OpenAlex.
- The conserved human astrovirus-receptor interface reveals a targetable vulnerability for antiviral development.Nature communications · 2026Article
- Article
- Chicken caspase-3 promotes IBDV replication via the cleavage of IRF7.Frontiers in microbiology · 2026Article
- Distinct intracellular and extracellular maturation of goose astrovirus 2 capsid protein and the potential of virus-like particles as a novel subunit vaccine.Frontiers in veterinary science · 2026Article
- Structure of the human astrovirus capsid spike in complex with the neonatal Fc receptor.Nature communications · 2025Article
- Nonlytic Egress and Transmission in the Virus World.Annual review of biochemistry · 2025Review
- Host proteases: key regulators in viral infection and therapeutic targeting.Frontiers in immunology · 2025Review
- Neonatal Fc receptor is a functional receptor for classical human astrovirus.Genes to cells : devoted to molecular & cellular mechanisms · 2024Article
- Structure and antigenicity of the divergent human astrovirus VA1 capsid spike.PLoS pathogens · 2024Article
- Attenuation hotspots in neurotropic human astroviruses.PLoS biology · 2023Article
- Structure of the divergent human astrovirus MLB capsid spike.Structure (London, England : 1993) · 2022Article
- The Association of Human Astrovirus with Extracellular Vesicles Facilitates Cell Infection and Protects the Virus from Neutralizing Antibodies.Journal of virology · 2022Article
- The Capsid Precursor Protein of Astrovirus VA1 Is Proteolytically Processed Intracellularly.Journal of virology · 2022Article
- An Insight into the Molecular Characteristics and Associated Pathology of Chicken Astroviruses.Viruses · 2022Review
- Structures of Two Human Astrovirus Capsid/Neutralizing Antibody Complexes Reveal Distinct Epitopes and Inhibition of Virus Attachment to Cells.Journal of virology · 2022Article
- Structural Insights into the Human Astrovirus Capsid.Viruses · 2021Review
- Egress of non-enveloped enteric RNA viruses.The Journal of general virology · 2021Review
- Protein Disulfide Isomerase A4 Is Involved in Genome Uncoating during Human Astrovirus Cell Entry.Viruses · 2020Article
- Nonsuppurative (Aseptic) Meningoencephalomyelitis Associated with Neurovirulent Astrovirus Infections in Humans and Animals.Clinical microbiology reviews · 2018Review
- The Ubiquitin-Proteasome System Is Necessary for Efficient Replication of Human Astrovirus.Journal of virology · 2018Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In this work we have shown that astrovirus infection induces apoptosis of Caco-2 cells, since fragmentation of cellular DNA, cleavage of cellular proteins which are substrate of activated caspases, and a change in the mitochondrial transmembrane potential occur upon virus infection. The human astrovirus Yuc8 polyprotein capsid precursor VP90 is initially processed to yield VP70, and we have shown that this processing is trypsin independent and occurs intracellularly through four cleavages at its carboxy-terminal region. We further showed that VP90-VP70 processing is mediated by caspases, since it was blocked by the pancaspase inhibitor benzyloxycarbonyl-Val-Ala-Asp fluoromethylketone (z-VAD-fmk), and it was promoted by the apoptosis inducer TNF-related apoptosis-inducing ligand (TRAIL). Although the cell-associated virus produced in the presence of these compounds was not affected, the release of infectious virus to the cell supernatant was drastically reduced in the presence of z-VAD-fmk and increased by TRAIL, indicating that VP90-VP70 cleavage is important for the virus particles to be released from the cell. This is the first report that describes the induction and utilization of caspase activity by a virus to promote processing of the capsid precursor and dissemination of the viral particles.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.