ArticleThe Journal of experimental medicine2004
Deletion of the nucleotide excision repair gene Ercc1 reduces immunoglobulin class switching and alters mutations near switch recombination junctions.
Article in The Journal of experimental medicine, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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Who cites it
18 citing papers in PubMed, 40 citations in OpenAlex.
- Antibody Deficiency in Xeroderma Pigmentosum.Journal of clinical immunology · 2026Article
- Nucleotide level mapping of uracils in murine heavy chain switch regions shows correlation between uracilation and positions of switch junctions created during class-switch recombination.NAR molecular medicine · 2025Article
- Alternative end-joining in BCR gene rearrangements and translocations.Acta biochimica et biophysica Sinica · 2022Review
- Review
- Article
- Chemoresistant lung cancer stem cells display high DNA repair capability to remove cisplatin-induced DNA damage.British journal of pharmacology · 2017Article
- Review
- Regulation of immunoglobulin class-switch recombination: choreography of noncoding transcription, targeted DNA deamination, and long-range DNA repair.Advances in immunology · 2014Review
- Cell autonomous and nonautonomous mechanisms drive hematopoietic stem/progenitor cell loss in the absence of DNA repair.Stem cells (Dayton, Ohio) · 2013Article
- The DSIF subunits Spt4 and Spt5 have distinct roles at various phases of immunoglobulin class switch recombination.PLoS genetics · 2012Article
- Review
- Excision repair cross-complementing 1 expression protects against ischemic injury following middle cerebral artery occlusion in the rat brain.Gene therapy · 2009Article
- ERCC1-XPF endonuclease facilitates DNA double-strand break repair.Molecular and cellular biology · 2008Article
- Mechanism and regulation of class switch recombination.Annual review of immunology · 2008Review
- Nucleotide excision repair in an immunoglobulin variable gene is less efficient than in a housekeeping gene.Molecular immunology · 2007Article
- Inducible DNA breaks in Ig S regions are dependent on AID and UNG.The Journal of experimental medicine · 2005Article
- B cells from hyper-IgM patients carrying UNG mutations lack ability to remove uracil from ssDNA and have elevated genomic uracil.The Journal of experimental medicine · 2005Article
- MSH2-MSH6 stimulates DNA polymerase eta, suggesting a role for A:T mutations in antibody genes.The Journal of experimental medicine · 2005Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
The structure-specific endonuclease ERCC1-XPF is an essential component of the nucleotide excision DNA repair pathway. ERCC1-XPF nicks double-stranded DNA immediately adjacent to 3' single-strand regions. Substrates include DNA bubbles and flaps. Furthermore, ERCC1 interacts with Msh2, a mismatch repair (MMR) protein involved in class switch recombination (CSR). Therefore, ERCC1-XPF has abilities that might be useful for antibody CSR. We tested whether ERCC1 is involved in CSR and found that Ercc1(-)(/)(-) splenic B cells show moderately reduced CSR in vitro, demonstrating that ERCC1-XPF participates in, but is not required for, CSR. To investigate the role of ERCC1 in CSR, the nucleotide sequences of switch (S) regions were determined. The mutation frequency in germline Smicro segments and recombined Smicro-Sgamma3 segments cloned from Ercc1(-)(/)(-) splenic B cells induced to switch in culture was identical to that of wild-type (WT) littermates. However, Ercc1(-)(/)(-) cells show increased targeting of the mutations to G:C bp in RGYW/WRCY hotspots and mutations occur at sites more distant from the S-S junctions compared with WT mice. The results indicate that ERCC1 is not epistatic with MMR and suggest that ERCC1 might be involved in processing or repair of DNA lesions in S regions during CSR.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.