ArticleGenetics2004
The distribution of transgene insertion sites in barley determined by physical and genetic mapping.
Article in Genetics, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 47 citations in OpenAlex.
- Fluorescence chromosome banding and FISH mapping in perennial ryegrass, Lolium perenne L.BMC genomics · 2016Article
- Current and new approaches in GMO detection: challenges and solutions.BioMed research international · 2015Review
- Analysis of T-DNA/Host-Plant DNA Junction Sequences in Single-Copy Transgenic Barley Lines.Biology · 2014Article
- Identification of "safe harbor" loci in indica rice genome by harnessing the property of zinc-finger nucleases to induce DNA damage and repair.Frontiers in plant science · 2014Article
- Analysis of chromosomal polymorphism in barley (Hordeum vulgare L. ssp. vulgare) and between H. vulgare and H. chilense using three-color fluorescence in situ hybridization (FISH).Journal of applied genetics · 2013Article
- Comparison of DNA walking methods for isolation of transgene-flanking regions in GM potato.Molecular biotechnology · 2011Article
- Characterisation of 3' transgene insertion site and derived mRNAs in MON810 YieldGard maize.Plant molecular biology · 2008Article
- In situ methods to localize transgenes and transcripts in interphase nuclei: a tool for transgenic plant research.Plant methods · 2006Article
- Unintended consequences of plant transformation: a molecular insight.Journal of applied genetics · 2006Review
- Introgression of wheat DNA markers from A, B and D genomes in early generation progeny of Aegilops cylindrica Host x Triticum aestivum L. hybrids.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2005Article
- Mammary specific transgenic over-expression of insulin-like growth factor-I (IGF-I) increases pig milk IGF-I and IGF binding proteins, with no effect on milk composition or yield.Transgenic research · 2005Article
- A comparison of transgenic barley lines produced by particle bombardment and Agrobacterium-mediated techniques.Plant cell reports · 2005Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The exact site of transgene insertion into a plant host genome is one feature of the genetic transformation process that cannot, at present, be controlled and is often poorly understood. The site of transgene insertion may have implications for transgene stability and for potential unintended effects of the transgene on plant metabolism. To increase our understanding of transgene insertion sites in barley, a detailed analysis of transgene integration in independently derived transgenic barley lines was carried out. Fluorescence in situ hybridization (FISH) was used to physically map 23 transgene integration sites from 19 independent barley lines. Genetic mapping further confirmed the location of the transgenes in 11 of these lines. Transgene integration sites were present only on five of the seven barley chromosomes. The pattern of transgene integration appeared to be nonrandom and there was evidence of clustering of independent transgene insertion events within the barley genome. In addition, barley genomic regions flanking the transgene insertion site were isolated for seven independent lines. The data from the transgene flanking regions indicated that transgene insertions were preferentially located in gene-rich areas of the genome. These results are discussed in relation to the structure of the barley genome.
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