ArticleBMC biotechnology2004
Investigating the utility of combining phi29 whole genome amplification and highly multiplexed single nucleotide polymorphism BeadArray genotyping.
Article in BMC biotechnology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed.
- Next-Generation Sequencing Workflow for NSCLC Critical Samples Using a Targeted Sequencing Approach by Ion Torrent PGM™ Platform.International journal of molecular sciences · 2015Article
- Lack of association between four SNPs in the SLC22A3-LPAL2-LPA gene cluster and coronary artery disease in a Chinese Han population: a case control study.Lipids in health and disease · 2012Article
- Genotyping performance assessment of whole genome amplified DNA with respect to multiplexing level of assay and its period of storage.PloS one · 2011Article
- Article
- Whole-genome amplification enables accurate genotyping for microarray-based high-density single nucleotide polymorphism array.Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology · 2008Article
- Coupling amplified DNA from flow-sorted chromosomes to high-density SNP mapping in barley.BMC genomics · 2008Article
- Performance of whole-genome amplified DNA isolated from serum and plasma on high-density single nucleotide polymorphism arrays.The Journal of molecular diagnostics : JMD · 2008Article
- SNP high-throughput screening in grapevine using the SNPlex genotyping system.BMC plant biology · 2008Article
- Large fragment Bst DNA polymerase for whole genome amplification of DNA from formalin-fixed paraffin-embedded tissues.BMC genomics · 2006Article
- Improvements of rolling circle amplification (RCA) efficiency and accuracy using Thermus thermophilus SSB mutant protein.Nucleic acids research · 2006Article
- Effects of DNA mass on multiple displacement whole genome amplification and genotyping performance.BMC biotechnology · 2005Article
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Authors and funding
12 authors.
Funding
Abstract
backgroundSustainable DNA resources and reliable high-throughput genotyping methods are required for large-scale, long-term genetic association studies. In the genetic dissection of common disease it is now recognised that thousands of samples and hundreds of thousands of markers, mostly single nucleotide polymorphisms (SNPs), will have to be analysed. In order to achieve these aims, both an ability to boost quantities of archived DNA and to genotype at low costs are highly desirable. We have investigated phi29 polymerase Multiple Displacement Amplification (MDA)-generated DNA product (MDA product), in combination with highly multiplexed BeadArray genotyping technology. As part of a large-scale BeadArray genotyping experiment we made a direct comparison of genotyping data generated from MDA product with that from genomic DNA (gDNA) templates.
resultsEighty-six MDA product and the corresponding 86 gDNA samples were genotyped at 345 SNPs and a concordance rate of 98.8% was achieved. The BeadArray sample exclusion rate, blind to sample type, was 10.5% for MDA product compared to 5.8% for gDNA.
conclusionsWe conclude that the BeadArray technology successfully produces high quality genotyping data from MDA product. The combination of these technologies improves the feasibility and efficiency of mapping common disease susceptibility genes despite limited stocks of gDNA samples.
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