ArticleExtremophiles : life under extreme conditions2004
Engineering the thermostability of Trichoderma reesei endo-1,4-beta-xylanase II by combination of disulphide bridges.
Article in Extremophiles : life under extreme conditions, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed.
- Three Molecular Modification Strategies to Improve the Thermostability of Xylanase XynA fromFoods (Basel, Switzerland) · 2023Article
- Inhibitory effect of lignin on the hydrolysis of xylan by thermophilic and thermolabile GH11 xylanases.Biotechnology for biofuels and bioproducts · 2022Article
- DSDBASE 2.0: updated version of DiSulphide dataBASE, a database on disulphide bonds in proteins.Database : the journal of biological databases and curation · 2022Article
- Extremophilic Prokaryotic Endoxylanases: Diversity, Applicability, and Molecular Insights.Frontiers in microbiology · 2021Review
- Hyperthermostable Thermotoga maritima xylanase XYN10B shows high activity at high temperatures in the presence of biomass-dissolving hydrophilic ionic liquids.Extremophiles : life under extreme conditions · 2016Article
- Thermostable microbial xylanases for pulp and paper industries: trends, applications and further perspectives.World journal of microbiology & biotechnology · 2016Review
- Thermal behaviour and tolerance to ionic liquid [emim]OAc in GH10 xylanase from Thermoascus aurantiacus SL16W.Extremophiles : life under extreme conditions · 2014Article
- Effect of Temperature on Xylanase II from Trichoderma reesei QM 9414: A Calorimetric, Catalytic, and Conformational Study.Enzyme research · 2014Article
- Article
- Introduction of a disulfide bridge enhances the thermostability of a Streptomyces olivaceoviridis xylanase mutant.Journal of industrial microbiology & biotechnology · 2007Article
- Characterization of proteolytic bacteria from the Aleutian deep-sea and their proteases.Journal of industrial microbiology & biotechnology · 2007Article
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4 authors.
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Abstract
Disulphide bridges were introduced in different combinations into the N-terminal region and the single alpha-helix of mesophilic Trichoderma reesei xylanase II (TRX II). We used earlier disulphide-bridge data and designed new disulphide bridges for the combination mutants. The most stable mutant contained two disulphide bridges (between positions 2 and 28 and between positions 110 and 154, respectively) and the mutations N11D, N38E, and Q162H. With a half-life of approximately 56 h at 65 degrees C, the thermostability of this sevenfold mutant was approximately 5,000 times higher than that of TRX II, and the half-life was 25 min even at 75 degrees C. The thermostability of this mutant was approximately 30 times higher than that of the corresponding mutant missing the bridge between positions 2 and 28. The extensive stabilization at two protein regions did not alter the kinetic properties of the sevenfold mutant from that of the wild-type TRX II. The combination of disulphide bridges enhanced significantly the pH-dependent stability in a wide pH range.
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