ArticleCurrent genetics2004
Expression studies of GUP1 and GUP2, genes involved in glycerol active transport in Saccharomyces cerevisiae, using semi-quantitative RT-PCR.
Article in Current genetics, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
What it found
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Who cites it
6 citing papers in PubMed, 23 citations in OpenAlex.
- Advances in glycerol metabolism comprehension in yeasts: from regulation to metabolic engineering strategies.World journal of microbiology & biotechnology · 2026Review
- Yeast Gup1(2) Proteins Are Homologues of the Hedgehog Morphogens Acyltransferases HHAT(L): Facts and Implications.Journal of developmental biology · 2016Review
- Pheromone-induced morphogenesis improves osmoadaptation capacity by activating the HOG MAPK pathway.Science signaling · 2013Article
- Saccharomyces cerevisiae glycerol/H+ symporter Stl1p is essential for cold/near-freeze and freeze stress adaptation. A simple recipe with high biotechnological potential is given.Microbial cell factories · 2010Article
- A member of the sugar transporter family, Stl1p is the glycerol/H+ symporter in Saccharomyces cerevisiae.Molecular biology of the cell · 2005Article
- Arabidopsis POLYOL TRANSPORTER5, a new member of the monosaccharide transporter-like superfamily, mediates H+-Symport of numerous substrates, including myo-inositol, glycerol, and ribose.The Plant cell · 2005Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Glycerol active uptake in Saccharomyces cerevisiae, characterised physiologically as a proton symport, was previously described as repressed by glucose, induced by growth on non-fermentable carbon sources and unresponsive to growth under salt stress. GUP1 and GUP2 were identified and characterised as genes involved in glycerol active uptake. Using semi-quantitative RT-PCR, GUP1 and GUP2 transcription was measured. Unlike active transport activity determined previously, this was shown to be constitutive and not affected by either glucose repression or growth under salt stress. Furthermore, transcription of GUP1 and GUP2 was not affected in the gpd1gpd2 mutant strain grown under salt stress in the presence of small amounts of glycerol, in which case a very high Vmax of glycerol uptake was reported. Intracellular compounds were determined. Glycerol, acetate and trehalose were found to be the major compounds accumulated. Surprisingly, the gpd1gpd2 mutant was found to produce significant amounts of glycerol. Yet, the results provide no evidence for a correlation between the amount of each compound and the glycerol transport activity in any of the strains.
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Registered trials
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