ArticleProceedings of the National Academy of Sciences of the United States of America2004
Discriminatory aptamer reveals serum response element transcription regulated by cytohesin-2.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 63 citations in OpenAlex.
- Therapeutic Potential of Aptamer-Protein Interactions.ACS pharmacology & translational science · 2022Review
- An overview and future prospects on aptamers for food safety.Applied microbiology and biotechnology · 2020Review
- Aptamers as Valuable Molecular Tools in Neurosciences.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2017Review
- Selective aptamer-based control of intraneuronal signaling.Angewandte Chemie (International ed. in English) · 2015Article
- Isolation of single-stranded DNA aptamers that distinguish influenza virus hemagglutinin subtype H1 from H5.PloS one · 2015Article
- Rapid identification of cell-specific, internalizing RNA aptamers with bioinformatics analyses of a cell-based aptamer selection.PloS one · 2012Article
- Aldolase directly interacts with ARNO and modulates cell morphology and acidic vesicle distribution.American journal of physiology. Cell physiology · 2011Article
- Cell-specific aptamers as emerging therapeutics.Journal of nucleic acids · 2011Article
- Aptamers as therapeutics.Nature reviews. Drug discovery · 2010Review
- Peripheral nerve injury up-regulates expression of interactor protein for cytohesin exchange factor 1 (IPCEF1) mRNA in rat dorsal root ganglion.Naunyn-Schmiedeberg's archives of pharmacology · 2009Article
- Aptamers selected against the unglycosylated EGFRvIII ectodomain and delivered intracellularly reduce membrane-bound EGFRvIII and induce apoptosis.Biological chemistry · 2009Article
- An RNA molecule that specifically inhibits G-protein-coupled receptor kinase 2 in vitro.RNA (New York, N.Y.) · 2008Article
- The N-terminal coiled-coil domain of the cytohesin/ARNO family of guanine nucleotide exchange factors interacts with Galphaq.Molecular and cellular biochemistry · 2007Article
- Molecular tools for cell and systems biology.HFSP journal · 2007Article
- Delivery systems for in vivo use of nucleic Acid drugs.Drug target insights · 2007Article
- RNA aptamers selectively modulate protein recruitment to the cytoplasmic domain of beta-secretase BACE1 in vitro.RNA (New York, N.Y.) · 2006Article
- Analysis of bronchoalveolar lavage fluid proteome from systemic sclerosis patients with or without functional, clinical and radiological signs of lung fibrosis.Arthritis research & therapy · 2006Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cytohesins are a family of highly homologous guanine nucleotide exchange factors (GEFs) that act on ADP-ribosylation factors (ARFs). The small ARF-GEFs are involved in integrin signaling, actin cytoskeleton remodeling, and vesicle transport. Here, we selected and applied a specific inhibitor for ARF nucleotide-binding site opener (ARNO)/cytohesin-2, an RNA aptamer that clearly discriminates between cytohesin-1 and cytohesin-2. This reagent bound to an N-terminal segment of cytohesin-2 and did not inhibit ARF-GEF function in vitro. When transfected into HeLa cells, it persisted for at least 6 h without requiring stabilization. Its effect in vivo was to down-regulate gene expression mediated through the serum-response element and knockdown mitogen-activated protein kinase activation, indicating that cytohesin-2 acts by means of mitogen-activated protein kinase signaling. We conclude that the N-terminal coiled-coil and parts of the Sec7 domain of cytohesin-2 are required for serum-mediated transcriptional activation in nonimmune cells, whereas cytohesin-1 is not. Our results indicate that intramer technology can be used not only for assigning novel biological functions to proteins or protein domains but also to prove nonredundancy of highly homologous proteins.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.