ArticleProceedings of the National Academy of Sciences of the United States of America2004
Bioreactor-based bone tissue engineering: the influence of dynamic flow on osteoblast phenotypic expression and matrix mineralization.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 68 papers.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
68 citing papers in PubMed, 295 citations in OpenAlex.
- Engineering Multiscale Vasculature: Biological Principles, Design Constraints, and Advanced Biofabrication Strategies for Functional Vascular Networks.Biomimetics (Basel, Switzerland) · 2026Review
- Osteoinductive low-dose 3D porous calcium phosphate graphene oxide-integrated matrices enhance osteogenesis and mechanical properties.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Graphene Oxide in Bone Regenerative Engineering: Current Challenges and Future Perspectives.ACS bio & med chem Au · 2025Review
- Magnesium phosphate functionalized graphene oxide and PLGA composite matrices with enhanced mechanical and osteogenic properties for bone regeneration.Regenerative biomaterials · 2025Article
- Classes of Stem Cells: From Biology to Engineering.Regenerative engineering and translational medicine · 2024Article
- Animal Models of Osteoarthritis: Updated Models and Outcome Measures 2016-2023.Regenerative engineering and translational medicine · 2024Article
- Hyaluronic acid-British anti-Lewisite as a safer chelation therapy for the treatment of arthroplasty-related metallosis.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Perfusion of MC3T3E1 Preosteoblast Spheroids within Polysaccharide-Based Hydrogel Scaffolds: An Experimental and Numerical Study at the Bioreactor Scale.Bioengineering (Basel, Switzerland) · 2023Article
- Osteochondral regenerative engineering: challenges, state-of-the-art and translational perspectives.Regenerative biomaterials · 2023Review
- Translation of biophysical environment in bone into dynamic cell culture under flow for bone tissue engineering.Computational and structural biotechnology journal · 2023Review
- Generation and evaluation of input values for computational analysis of transport processes within tissue cultures.Engineering in life sciences · 2022Review
- Scaffolding Biomaterials for 3D Cultivated Meat: Prospects and Challenges.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022Review
- 3D Tissue-Engineered Vascular Drug Screening Platforms: Promise and Considerations.Frontiers in cardiovascular medicine · 2022Review
- Evaluation of bone formation on orthopedic implant surfaces using an ex-vivo bone bioreactor system.Scientific reports · 2021Article
- Designing and validation of an automated ex-vivo bioreactor system for long term culture of bone.Bone reports · 2021Article
- Vascular Tissue Engineering: Challenges and Requirements for an Ideal Large Scale Blood Vessel.Frontiers in bioengineering and biotechnology · 2021Review
- Low-intensity vibration restores nuclear YAP levels and acute YAP nuclear shuttling in mesenchymal stem cells subjected to simulated microgravity.NPJ microgravity · 2020Article
- Controlled release of soy isoflavones from multifunctional 3D printed bone tissue engineering scaffolds.Acta biomaterialia · 2020Article
- Fluid flow-induced cell stimulation in bone tissue engineering changes due to interstitial tissue formation in vitro.International journal for numerical methods in biomedical engineering · 2020Article
- New generation of bioreactors that advance extracellular matrix modelling and tissue engineering.Biotechnology letters · 2019Review
8 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
Abstract
An important issue in tissue engineering concerns the possibility of limited tissue ingrowth in tissue-engineered constructs because of insufficient nutrient transport. We report a dynamic flow culture system using high-aspect-ratio vessel rotating bioreactors and 3D scaffolds for culturing rat calvarial osteoblast cells. 3D scaffolds were designed by mixing lighter-than-water (density, <1g/ml) and heavier-than-water (density, >1g/ml) microspheres of 85:15 poly(lactide-co-glycolide). We quantified the rate of 3D flow through the scaffolds by using a particle-tracking system, and the results suggest that motion trajectories and, therefore, the flow velocity around and through scaffolds in rotating bioreactors can be manipulated by varying the ratio of heavier-than-water to lighter-than-water microspheres. When rat primary calvarial cells were cultured on the scaffolds in bioreactors for 7 days, the 3D dynamic flow environment affected bone cell distribution and enhanced cell phenotypic expression and mineralized matrix synthesis within tissue-engineered constructs compared with static conditions. These studies provide a foundation for exploring the effects of dynamic flow on osteoblast function and provide important insight into the design and optimization of 3D scaffolds suitable in bioreactors for in vitro tissue engineering of bone.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.